A closed-loop strategy for endoglucanase production using sugarcane bagasse liquefied by a home-made enzymatic cocktail

Autor(es)

Squinca, Paula ^{[1, 2]} ; Badino, Alberto C. ^[1] ; Farinas, Cristiane S. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Grad Program Chem Engn, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[2] Embrapa Instrumentat, Rua 15 Novembro 1452, BR-13561206 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 249, p. 976-982, FEB 2018.
• Citações Web of Science: 3

Resumo

Use of the same lignocellulosic biomass as feedstock for enzymes and ethanol production has been suggested as a lower cost option in future biorefineries. Here, we propose a closed-loop strategy to produce the cellulolytic enzymes required for biomass hydrolysis using sugarcane bagasse liquefied by a home-made enzymatic cocktail as carbon source and inducer. The fed-batch liquefaction conditions were firstly evaluated using commercial enzymes. Subsequently, the effects of different liquefied materials and solids loadings on endoglucanase production by Aspergillus niger cultivated in submerged fermentation were investigated. The liquefied bagasse produced using the home-made cocktail was more favorable for endoglucanase production, resulting in improvement up to 17%, compared to bagasse liquefied by commercial enzymes. The results indicated that liquefied bagasse produced by home-made enzymatic cocktail could provide a cost-effective carbon source and inducer for cellulolytic enzyme production, and could contribute to closing loops within the biorefinery, thus reducing costs and minimizing waste. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 14/19000-3 - Simplificação do processo de conversão de biomassa a etanol por meio da integração da produção de enzimas in-house
• Beneficiário: Cristiane Sanchez Farinas
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular