Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A closed-loop strategy for endoglucanase production using sugarcane bagasse liquefied by a home-made enzymatic cocktail

Full text
Squinca, Paula [1, 2] ; Badino, Alberto C. [1] ; Farinas, Cristiane S. [1, 2]
Total Authors: 3
[1] Univ Fed Sao Carlos, Grad Program Chem Engn, CP 676, BR-13565905 Sao Carlos, SP - Brazil
[2] Embrapa Instrumentat, Rua 15 Novembro 1452, BR-13561206 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Bioresource Technology; v. 249, p. 976-982, FEB 2018.
Web of Science Citations: 1

Use of the same lignocellulosic biomass as feedstock for enzymes and ethanol production has been suggested as a lower cost option in future biorefineries. Here, we propose a closed-loop strategy to produce the cellulolytic enzymes required for biomass hydrolysis using sugarcane bagasse liquefied by a home-made enzymatic cocktail as carbon source and inducer. The fed-batch liquefaction conditions were firstly evaluated using commercial enzymes. Subsequently, the effects of different liquefied materials and solids loadings on endoglucanase production by Aspergillus niger cultivated in submerged fermentation were investigated. The liquefied bagasse produced using the home-made cocktail was more favorable for endoglucanase production, resulting in improvement up to 17%, compared to bagasse liquefied by commercial enzymes. The results indicated that liquefied bagasse produced by home-made enzymatic cocktail could provide a cost-effective carbon source and inducer for cellulolytic enzyme production, and could contribute to closing loops within the biorefinery, thus reducing costs and minimizing waste. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 14/19000-3 - Simplification of the biomass to ethanol conversion process by integration with the production of enzymes in-house
Grantee:Cristiane Sanchez Farinas
Support type: Program for Research on Bioenergy (BIOEN) - Regular Program Grants