Sweet Vector for Gene Delivery: the Sugar Decoration of Polyplexes Reduces Cytotoxicity with a Balanced Effect on Gene Expression

Autor(es)

Albuquerque, Lindomar J. C. ^[1] ; Alavarse, Alex C. ^[1] ; Carlan da Silva, Maria C. ^[1] ; Zilse, Morgana S. ^[2] ; Barth, Maite T. ^[2] ; Bellettini, Ismael C. ^[2] ; Giacomelli, Fernando C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre - Brazil

^[2] Univ Fed Santa Catarina, Dept Ciencias Exatas & Educ, BR-89036256 Blumenau - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Macromolecular Bioscience; v. 18, n. 2 FEB 2018.
• Citações Web of Science: 3

Resumo

The use of sugar-functionalized polyplexes as a nonviral gene delivery vector with lower cytotoxicity than the well-known polymeric carrier branched polyethyleneimine (BPEI) is investigated. The substitution of primary amine groups in the BPEI chains with lactose residues leads to larger polyplexes, presumably due to the higher amount of polymer required to complete DNA condensation. Nevertheless, the sugar functionalization substantially reduces the cytotoxicity of the assemblies. The nanocomplexes are taken up by the cells to a greater extent, whereas the levels of gene expression are maintained compared to those obtained using BPEI, which is known for its excellent transfection efficiency. Accordingly, the preparation of lower-cytotoxicity polyplexes while maintaining gene expression, which is highly relevant to the field, is demonstrated. (AU)

• Processo FAPESP: 16/23844-8 - Vesículas poliméricas biodegradáveis e sensíveis ao pH para encapsulação de BIOMACROMOLÉCULAS
• Beneficiário: Lindomar Jose Calumby Albuquerque
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/00459-4 - Interações biofisicoquímicas na nanobiointerface: a influência das características superficiais e da presença de coroa protéica no processo de internalização celular de nanomateriais
• Beneficiário: Fernando Carlos Giacomelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular