Fluconazole impacts the extracellular matrix of fluconazole-susceptible and-resistant Candida albicans and Candida glabrata biofilms

Autor(es)

Dias Panariello, Beatriz Helena ^[1] ; Klein, I, Marlise ; De Oliveira Mima, Ewerton Garcia ^[2] ; Pavarina, Ana Claudia ^[2]

Afiliação do(s) autor(es)

^[1] Indiana Univ Purdue Univ, Sch Dent, Dept Cariol Operat Dent & Dent Publ Hlth, Indianapolis, IN 46202 - USA

^[2] Klein, Marlise, I, Sao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, Unesp, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL MICROBIOLOGY; v. 10, n. 1 JUN 4 2018.
• Citações Web of Science: 6

Resumo

Background: Fluconazole (FLZ) is a drug commonly used for the treatment of Candida infections. However, -glucans in the extracellular matrices (ECMs) hinder FLZ penetration into Candida biofilms, while extracellular DNA (eDNA) contributes to the biofilm architecture and resistance. Methods: This study characterized biofilms of FLZ-sensitive (S) and -resistant (R) Candida albicans and Candida glabrata in the presence or absence of FLZ focusing on the ECM traits. Biofilms of C albicans American Type Culture Collection (ATCC) 90028 (CaS), C. albicans ATCC 96901 (CaR), C. glabrata ATCC 2001 (CgS), and C. glabrata ATCC 200918 (CgR) were grown in RPM! medium with or without FLZ at 5x the minimum inhibitory concentration (37 degrees C/48 h). Biofilms were assessed by colony-forming unit (CFU)/mL, biomass, and ECM components (alkali-soluble polysaccharides {[}ASP], water-soluble polysaccharides {[}WSP], eDNA, and proteins). Scanning electron microscopy (SEM) was also performed. Data were analyzed by parametric and nonparametric tests (a = 0.05). Results: In biofilms, FLZ reduced the CFU/mL of all strains (p < 0.001), except for CaS (p = 0.937). However, the ASP quantity in CaS was significantly reduced by FLZ (p = 0.034), while the drug had no effect on the ASP levels in other strains (p > 0.05). Total biomasses and WSP were significantly reduced by FLZ in the ECM of all yeasts (p < 0.001), but levels of eDNA and proteins were unaffected (p > 0.05). FLZ affected the cell morphology and biofilm structure by hindering hyphae formation in CaS and CaR biofilms, by decreasing the number of cells in CgS and CgR biofilms, and by yielding sparsely spaced cell agglomerates on the substrate. Conclusion: FLZ impacts biofilms of C. albicans and C. glabrata as evident by reduced biomass. This reduced biomass coincided with lowered cell numbers and quantity of WSPs. Hyphal production by C. albicans was also reduced. (AU)

• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/18804-1 - Matriz extracelular de biofilmes de Candida albicans e Candida glabrata susceptíveis e resistentes ao fluconazol: Caracterização, ação de enzimas hidrolíticas e eficácia da associação das enzimas à Terapia Fotodinâmica.
• Beneficiário: Beatriz Helena Dias Panariello
• Modalidade de apoio: Bolsas no Brasil - Doutorado