Real-time PCR-based method for rapid detection of Aspergillus niger and Aspergillus welwitschiae isolated from coffee

Autor(es)

von Hertwig, Aline Morgan ^{[1, 2]} ; Sant'Ana, Anderson S. ^[2] ; Sartori, Daniele ^[3] ; da Silva, Josue Jose ^[3] ; Nascimento, Maristela S. ^[4] ; Iamanaka, Beatriz Thie ^[1] ; Pelegrinelli Fungaro, Maria Helena ^[3] ; Taniwaki, Marta Hiromi ^[1]

Afiliação do(s) autor(es)

^[1] Food Technol Inst ITAL, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, Campinas, SP - Brazil

^[3] Univ Estadual Londrina, Londrina, PR - Brazil

^[4] Univ Estadual Campinas, Fac Food Engn, Dept Food Technol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Microbiological Methods; v. 148, p. 87-92, MAY 2018.
• Citações Web of Science: 2

Resumo

Some species from Aspergillus section Nigri are morphologically very similar and altogether have been called A. niger aggregate. Although the species included in this group are morphologically very similar, they differ in their ability to produce mycotoxins and other metabolites and their taxonomical status has evolved continuously. Among them, A. niger and A. welwitschiae are ochratoxin A and fumonisin B-2 producers and their detection and/or identification is of crucial importance for food safety. The aim of this study was the development of a real-time PCR-based method for simultaneous discrimination of A. niger and A. welwitschiae from other species of the A. niger aggregate isolated from coffee beans. One primer pair and a hybridization probe specific for detection of A. niger and A. welwitschiae strains were designed based on the BenA gene sequences, and used in a Real-time PCR assay for the rapid discrimination between both these species from all others of the A. niger aggregate. The Real-time PCR assay was shown to be 100% efficient in discriminating the 73 isolates of A. niger/A. welwitschiae from the other A. niger aggregate species analyzed as a negative control. This result testifies to the use of this technique as a good tool in the rapid detection of these important toxigenic species. (AU)

• Processo FAPESP: 11/50136-0 - Biodiversidade das espécies toxigênicas de Aspergillus no Brasil: ocorrência, taxonomia polifásica e distribuição
• Beneficiário: Marta Hiromi Taniwaki
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular