Biocatalysts based on nanozeolite-enzyme complexes: Effects of alkoxysilane surface functionalization and biofuel production using microalgae lipids feedstock

Autor(es)

de Vasconcellos, Adriano ^[1] ; Miller, Alex Henrique ^[1] ; Aranda, Donato A. G. ^[2] ; Nery, Jose Geraldo ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Phys Dept, Lab Clean Energy Technol LACET, Campus Sao Jose do Rio Preto, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Sch Chem, Greentec Lab, BR-21941972 Rio De Janeiro, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 165, p. 150-157, MAY 1 2018.
• Citações Web of Science: 4

Resumo

Nanozeolites with different crystallographic structures (Nano/TS1, Nano/GIS, Nano/LTA, Nano/BEA, Nano/X, and Nano-X/Ni), functionalized with (3-aminopropyl)trimethoxysilane (APTMS) and crosslinked with glutaraldehyde (GA), were studied as solid supports for Thermomyces lanuginosus lipase (TLL) immobilization. Physicochemical characterizations of the surface-functionalized nanozeolites and nanozeolite-enzyme complexes were performed using XRD, SEM, AFM, ATR-FTIR, and zeta potential measurements. The experimental enzymatic activity results indicated that the nanozeolitic supports functionalized with APTMS and GA immobilized larger amounts of enzymes and provided higher enzymatic activities, compared to unfunctionalized supports. Correlations were observed among the nanozeolite surface charges, the enzyme immobilization efficiencies, and the biocatalyst activities. The catalytic performance and reusability of these enzyme-nanozeolite complexes were evaluated in the ethanolysis transesterification of microalgae oil to fatty acid ethyl esters (FAEEs). TLL immobilized on the nanozeolite supports functionalized with APTMS and GA provided the most efficient biocatalysis, with FAEEs yields above 93% and stability during five reaction cycles. Lower FAEEs yields and poorer catalytic stability were found for nanozeolite-enzyme complexes prepared only by physical adsorption. The findings indicated the viability of designing highly efficient biocatalysts for biofuel production by means of chemical modulation of nanozeolite surfaces. The high biocatalyst catalytic efficiency observed in ethanolysis reactions using a lipid feedstock that does not compete with food production is an advantage that should encourage the industrial application of these biocatalysts. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/24259-0 - Moléculas conjugadas peptídeo/quitosana com potencial farmacológico: síntese, prospecção de atividade em sistemas miméticos de membrana e avaliações em células
• Beneficiário: Marcia Perez dos Santos Cabrera
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/10092-4 - Nanozeólitas como suportes sólidos para imobilização enzimática: Síntese, caracterização de complexos nanozeólitas/enzimas e sua aplicação como catalisadores heterogêneos para produção de biodiesel via rota etílica
• Beneficiário: Adriano de Vasconcellos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/51851-5 - Síntese e caracterização de catalisadores heterogêneos a base de complexos nanozeolitas/enzimas e sua aplicação na produção de biodiesel via Rota Etílica usando como matérias-primas óleo de palma
• Beneficiário: Jose Geraldo Nery
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/24303-0 - Síntese e caracterização de novos biocatalisadores formados por complexos de nanozeólitas e enzimas lacases: suas aplicações em reações de oxidação do glicerol a compostos ácido tartrônico e ácido mesoxálico
• Beneficiário: Alex Henrique Miller
• Modalidade de apoio: Bolsas no Brasil - Doutorado