Laccase production in bioreactor scale under saline condition by the marine-derived basidiomycete Peniophora sp CBMAI 1063

Autor(es)

Mainardi, Pedro H. ^[1] ; Feitosa, Valker A. ^[2] ; Brenelli de Paiva, Livia B. ^[3] ; Bonugli-Santos, Rafaella C. ^[4] ; Squina, Fabio M. ^[5] ; Pessoa, Jr., Adalberto ^[2] ; Sette, Lara D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Bioquim & Microbiol, Rio Claro, SP - Brazil

^[2] Univ Sao Paulo, FCF, Sao Paulo, SP - Brazil

^[3] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Campinas, SP - Brazil

^[4] Univ Fed Integracao Latino Amer UNICA, Inst Latino Amer Ciencias Vida, Parana, PR - Brazil

^[5] Univ Sorocaba, Programa Proc Tecnol & Ambientais, Sorocaba - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FUNGAL BIOLOGY; v. 122, n. 5, p. 302-309, MAY 2018.
• Citações Web of Science: 8

Resumo

Laccase production in saline conditions is still poorly studied. The aim of the present study was to investigate the production of laccase in two different types of bioreactors by the marine-derived basidiomycete Peniophora sp. CBMAI 1063. The highest laccase activity and productivity were obtained in the Stirred Tank (ST) bioreactor, while the highest biomass concentration in Air-lift (AL) bioreactor. The main laccase produced was purified by ion exchange and size exclusion chromatography and appeared to be monomeric with molecular weight of approximately 55 kDa. The optimum oxidation activity was obtained at pH 5.0. The thermal stability of the enzyme ranged from 30 to 50 degrees C (120 min). The Far-UV Circular Dichroism revealed the presence of high beta-sheet and low a-helical conformation in the protein structure. Additional experiments carried out in flask scale showed that the marine-derived fungus was able to produce laccase only in the presence of artificial seawater and copper sulfate. Results from the present study confirmed the fungal adaptation to marine conditions and its potential for being used in saline environments and/or processes. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/06923-6 - Recalcitrância da biomassa de cana-de-açúcar: fundamentos relacionados à formação da parede celular, ao pré-tratamento e à digestão enzimática, aplicados no desenvolvimento de novos modelos de biorrefinarias
• Beneficiário: Andre Luis Ferraz
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 13/12505-0 - Produção de lacases pelo fungo de origem marinha Peniophora sp. CBMAI 1063 em biorreator de bancada
• Beneficiário: Pedro Henrique Mainardi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/03061-0 - Caracterização de frações de lignina extraídas do bagaço de cana-de-açúcar com propriedades antioxidantes
• Beneficiário: Lívia Beatriz Brenelli de Paiva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/07957-7 - Micologia marinha e Antártica: diversidade e aplicação ambiental
• Beneficiário: Lara Durães Sette
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/19486-0 - Biotecnologia marinha e Antártica: enzimas microbianas e suas aplicações
• Beneficiário: Lara Durães Sette
• Modalidade de apoio: Auxílio à Pesquisa - Regular