Laccase production in bioreactor scale under saline condition by the marine-derived basidiomycete Peniophora sp CBMAI 1063

Author(s)

Mainardi, Pedro H. ^[1] ; Feitosa, Valker A. ^[2] ; Brenelli de Paiva, Livia B. ^[3] ; Bonugli-Santos, Rafaella C. ^[4] ; Squina, Fabio M. ^[5] ; Pessoa, Jr., Adalberto ^[2] ; Sette, Lara D. ^[1]

Affiliation

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Bioquim & Microbiol, Rio Claro, SP - Brazil

^[2] Univ Sao Paulo, FCF, Sao Paulo, SP - Brazil

^[3] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Campinas, SP - Brazil

^[4] Univ Fed Integracao Latino Amer UNICA, Inst Latino Amer Ciencias Vida, Parana, PR - Brazil

^[5] Univ Sorocaba, Programa Proc Tecnol & Ambientais, Sorocaba - Brazil

• Document type: Journal article
• Source: FUNGAL BIOLOGY; v. 122, n. 5, p. 302-309, MAY 2018.
• Web of Science Citations: 8

Abstract

Laccase production in saline conditions is still poorly studied. The aim of the present study was to investigate the production of laccase in two different types of bioreactors by the marine-derived basidiomycete Peniophora sp. CBMAI 1063. The highest laccase activity and productivity were obtained in the Stirred Tank (ST) bioreactor, while the highest biomass concentration in Air-lift (AL) bioreactor. The main laccase produced was purified by ion exchange and size exclusion chromatography and appeared to be monomeric with molecular weight of approximately 55 kDa. The optimum oxidation activity was obtained at pH 5.0. The thermal stability of the enzyme ranged from 30 to 50 degrees C (120 min). The Far-UV Circular Dichroism revealed the presence of high beta-sheet and low a-helical conformation in the protein structure. Additional experiments carried out in flask scale showed that the marine-derived fungus was able to produce laccase only in the presence of artificial seawater and copper sulfate. Results from the present study confirmed the fungal adaptation to marine conditions and its potential for being used in saline environments and/or processes. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 16/07957-7 - Marine and Antarctic Mycology: diversity and environmental application
• Grantee: Lara Durães Sette
• Support type: Regular Research Grants
• FAPESP's process: 13/03061-0 - Characterization and antioxidant capacity of fractions of lignin extracted from sugarcane bagasse
• Grantee: Lívia Beatriz Brenelli de Paiva
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models
• Grantee: Andre Luis Ferraz
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 13/12505-0 - Lacases production by marine derived fungi Peniophora sp. CBMAI 1063 on a bench bioreactor
• Grantee: Pedro Henrique Mainardi
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 13/19486-0 - Marine and Antarctic biotechnology: microbial enzymes and their applications
• Grantee: Lara Durães Sette
• Support type: Regular Research Grants