Characterization and antioxidant capacity of fractions of lignin extracted from sugarcane bagasse

• Grant number: 13/03061-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): July 01, 2013
• Effective date (End): June 30, 2017
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Fábio Márcio Squina
• Grantee: Lívia Beatriz Brenelli de Paiva
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovações (Brasil). Campinas , SP, Brazil
• Associated research grant: 08/58037-9 - Library generation for biomass-conversion enzymes from soil metagenome, AP.BIOEN.JP
• Associated scholarship(s): 15/07008-2 - Understanding the complex structure of lignin from sugarcane bagasse and the impact on the antioxidant capacity and its biochemical conversion into value added products, BE.EP.DR

Abstract

Antioxidants are molecules that have the capacity to inhibit oxidative reactions, which can result in deterioration of foods, nondurable goods and body cells. Therefore, the inhibition of oxidative reactions is of great interest in many industrial sectors, such as food, pharmaceutical, petrochemical, chemical and several industries related to human health. Polyphenols are substances that exhibit antioxidant capacity similar to synthetic antioxidants currently used in industries. Polyphenols have been extensively studied in recent years because of the possibility of obtaining these molecules from agro-industrial by-products. Sugarcane bagasse, a by-product derivated from ethanol and sugar process, is a lignocellulosic agro-industrial residue with high availability in Brazil, which can be employed for the production of second-generation ethanol and other value-added products. Due to the recalcitrant nature of lignocellulosic biomass, the pre-treatment is necessary to remove lignin, which turns the hemicellulose and cellulose available for subsequent enzymatic hydrolysis steps for biofuel production. Lignin is an amorphous macromolecule composed of phenylpropane units and represents above 20%-30% of the total lignocellulosic material. Its fragmentation generates phenolic compounds and polymers with different properties and functions of interest to several industrial sectors. Considering the global trend for replacement of non-renewable sources to renewable agro-industrial by-products, the use of antioxidants derived from lignin represents as a promising area of research. Here in this present proposal, we show preliminary results that report the antioxidant potential of lignin fragments extracted from sugarcane bagasse. Thus, this project aims to evaluate the antioxidant properties against reactive oxygen species (ROS) and nitrogen species (RNS) of the phenolic compounds present in lignin fractions derived from the sugarcane bagasse. The project also covers the fragmentation of lignin via chemical and enzymatic route, separation of lignin fragments by extraction with organic solvents; characterization of fractions obtained by mass spectrometry, infrared spectroscopy and nuclear magnetic resonance spectroscopy. The composition of the fractions will be correlated with antioxidant capacity against ROS and RNS through multivariate statistical analysis. In addition, we propose in this project the preparation of non-ionic emulsion based preparation with high antioxidant potential. This project will be developed at Brazilian Bioethanol Science and Technology Laboratory (CTBE) in partnership with the Department of Food Science, Faculty of Food Engineering in State University of Campinas. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRENELLI, LIVIA B.; PERSINOTI, GABRIELA F.; FRANCO CAIRO, JOAO PAULO L.; LIBERATO, MARCELO V.; GONCALVES, THIAGO AUGUSTO; OTERO, IGOR V. R.; MAINARDI, PEDRO H.; FELBY, CLAUS; SETTE, LARA D.; SQUINA, FABIO M.. Novel redox-active enzymes for ligninolytic applications revealed from multiomics analyses of Peniophora sp. CBMAI 1063, a laccase hyper-producer strain. SCIENTIFIC REPORTS, v. 9, NOV 26 2019. (13/03061-0, 13/19486-0, 15/07008-2, 14/04105-4, 16/09950-0, 15/06971-3, 15/50590-4, 17/16089-1)

MAINARDI, PEDRO H.; FEITOSA, VALKER A.; BRENELLI DE PAIVA, LIVIA B.; BONUGLI-SANTOS, RAFAELLA C.; SQUINA, FABIO M.; PESSOA, JR., ADALBERTO; SETTE, LARA D.. Laccase production in bioreactor scale under saline condition by the marine-derived basidiomycete Peniophora sp CBMAI 1063. FUNGAL BIOLOGY, v. 122, n. 5, p. 302-309, MAY 2018. (14/06923-6, 13/12505-0, 13/03061-0, 16/07957-7, 13/19486-0)

MANDELLI, F.; BRENELLI, L. B.; ALMEIDA, R. F.; GOLDBECK, R.; WOLF, L. D.; HOFFMAM, Z. B.; RULLER, R.; ROCHA, G. J. M.; MERCADANTE, A. Z.; SQUINA, F. M.. Simultaneous production of xylooligosaccharides and antioxidant compounds from sugarcane bagasse via enzymatic hydrolysis. INDUSTRIAL CROPS AND PRODUCTS, v. 52, p. 770-775, JAN 2014. (13/03061-0, 08/58037-9, 11/14200-6, 12/18859-5)

MORAES, EDUARDO C.; ALVAREZ, THABATA M.; PERSINOTI, GABRIELA F.; TOMAZETTO, GEIZECLER; BRENELLI, LIVIA B.; PAIXAO, DOUGLAS A. A.; EMATSU, GABRIELA C.; ARICETTI, JULIANA A.; CALDANA, CAMILA; DIXON, NEIL; et al. Lignolytic-consortium omics analyses reveal novel genomes and pathways involved in lignin modification and valorization. BIOTECHNOLOGY FOR BIOFUELS, v. 11, MAR 22 2018. (13/03061-0, 14/26152-4, 15/50590-4, 15/23279-6)

BRENELLI, LIVIA BEATRIZ; MARIUTTI, LILIAN REGINA BARROS; PORTUGAL, RODRIGO VILLARES; DE FARIAS, MARCELO ALEXANDRE; BRAGAGNOLO, NEURA; MERCADANTE, ADRIANA ZERLOTTI; FRANCO, TELMA TEIXEIRA; RABELO, SARITA CANDIDA; SQUINA, FABIO MARCIO. odified lignin from sugarcane bagasse as an emulsifier in oil-in-water nanoemulsion. INDUSTRIAL CROPS AND PRODUCTS, v. 167, SEP 1 2021. (13/03061-0, 20/05784-3, 15/50612-8, 15/50590-4)

TRAMONTINA, ROBSON; BRENELLI, LIVIA BEATRIZ; SOUSA, AMANDA; ALVES, RAFAEL; ZETTY ARENAS, ANA MARIA; NASCIMENTO, VIVIANE MARCOS; RABELO, SARITA CANDIDA; FREITAS, SINDELIA; RULLER, ROBERTO; SQUINA, FABIO MARCIO. Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms. Enzyme and Microbial Technology, v. 135, APR 2020. (15/50590-4, 13/03061-0, 17/15477-8, 15/26982-0, 16/07926-4)

FRANCO CAIRO, JOAO P. L.; CARAZZOLLE, MARCELO F.; LEONARDO, FLAVIA C.; MOFATTO, LUCIANA S.; BRENELLI, LIVIA B.; GONCALVES, THIAGO A.; UCHIMA, CRISTIANE A.; DOMINGUES, ROMANIA R.; ALVAREZ, THABATA M.; TRAMONTINA, ROBSON; et al. Expanding the Knowledge on Lignocellulolytic and Redox Enzymes of Worker and Soldier Castes from the Lower Termite Coptotermes gestroi. FRONTIERS IN MICROBIOLOGY, v. 7, OCT 13 2016. (15/06971-3, 14/20576-7, 14/50371-8, 12/19040-0, 14/10351-8, 11/20977-3, 13/03061-0, 10/11469-1, 08/58037-9)

BRENELLI, LIVIA BEATRIZ; MANDELLI, FERNANDA; MERCADANTE, ADRIANA ZERLOTTI; DE MORAES ROCHA, GEORGE JACKSON; ROCCO, SILVANA APARECIDA; CRAIEVICH, ALDO FELIX; GONCALVES, ADILSON ROBERTO; CENTENO, DANILO DA CRUZ; DE OLIVEIRA NETO, MARIO; SQUINA, FABIO MARCIO. Acidification treatment of lignin from sugarcane bagasse results in fractions of reduced polydispersity and high free-radical scavenging capacity. INDUSTRIAL CROPS AND PRODUCTS, v. 83, p. 94-103, MAY 2016. (13/03061-0, 14/50371-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PAIVA, Lívia Beatriz Brenelli de. Aplicação de abordagens químicas e biológicas para valorização da lignina. 2017. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.