Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms

Author(s)

Tramontina, Robson ^{[1, 2]} ; Brenelli, Livia Beatriz ^[1] ; Sousa, Amanda ^[1] ; Alves, Rafael ^[3] ; Zetty Arenas, Ana Maria ^{[1, 4]} ; Nascimento, Viviane Marcos ^[1] ; Rabelo, Sarita Candida ^[5] ; Freitas, Sindelia ^{[1, 4, 6]} ; Ruller, Roberto ^{[7, 8]} ; Squina, Fabio Marcio ^[9]

Affiliation

^[1] CNPEM, Lab Nacl Biorrenovaveis LNBR, Campinas, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Programa Biociencias & Tecnol Prod Bioativos BTPB, Campinas, SP - Brazil

^[3] Novozymes Amer Latina, Araucaria, PR - Brazil

^[4] Univ Estadual Campinas UNICAMP, Fac Engn Alimentos, Programa Bioenergia, Campinas, SP - Brazil

^[5] Univ Estadual Paulista Julio Mesquita Filho UNESP, Fac Ciencias Agron, Dept Bioproc & Biotecnol, Botucatu, SP - Brazil

^[6] Univ Estadual Campinas UNICAMP, FEQ, Campinas, SP - Brazil

^[7] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas IBILCE, Sao Jose Do Rio Preto, SP - Brazil

^[8] Univ Fed Mato Grosso do Sul, Inst Biociencias, Lab Bioquim Geral & Microorganismos, Campo Grande, MS - Brazil

^[9] Univ Sorocaba UNISO, Programa Proc Tecnol & Ambientais, Sorocaba, SP - Brazil

• Document type: Journal article
• Source: Enzyme and Microbial Technology; v. 135, APR 2020.
• Web of Science Citations: 0

Abstract

Bioproducts production using monomeric sugars derived from lignocellulosic biomass presents several challenges, such as to require a physicochemical pretreatment to improve its conversion yields. Hydrothermal lignocellulose pretreatment has several advantages and results in solid and liquid streams. The former is called hemicellulosic hydrolysate (HH), which contains inhibitory phenolic compounds and sugar degradation products that hinder microbial fermentation products from pentose sugars. Here, we developed and applied a novel enzyme process to detoxify HH. Initially, the design of experiments with different redox activities enzymes was carried out. The enzyme mixture containing the peroxidase (from Armoracia rusticana) together with superoxide dismutase (from Coptotermes gestroi) are the most effective to detoxify HH derived from sugarcane bagasse. Butanol fermentation by the bacteria Clostridium saccharoperbutylacetonicum and ethanol production by the yeast Scheffersomyces stipitis increased by 24.0 x and 2.4 x , respectively, relative to the untreated hemicellulosic hydrolysates. Detoxified HH was analyzed by chromatographic and spectrometric methods elucidating the mechanisms of phenolic compound modifications by enzymatic treatment. The enzyme mixture degraded and reduced the hydroxyphenyl- and feruloyl-derived units and polymerized the lignin fragments. This strategy uses biocatalysts under environmentally friendly conditions and could be applied in the fuel, food, and chemical industries. (AU)

• FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
• Grantee: Fábio Márcio Squina
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 13/03061-0 - Characterization and antioxidant capacity of fractions of lignin extracted from sugarcane bagasse
• Grantee: Lívia Beatriz Brenelli de Paiva
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/15477-8 - Development of a low severity pretreatment process for the production of sugarcane straw oligosaccharides
• Grantee: Lívia Beatriz Brenelli de Paiva
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 15/26982-0 - Exploring novel strategies for depolymerization of plant cell-wall polysaccharides: from structure, function and rational design of glycosyl hydrolases to biological implications and potential biotechnological applications
• Grantee: Mário Tyago Murakami
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 16/07926-4 - Novel integrative strategies through the aldoketo reductase from the termite Coptotermes gestroi for detoxification, saccharification and fermentation of lignocellulose aiming bioethanol production
• Grantee: Robson Tramontina
• Support type: Scholarships in Brazil - Doctorate