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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms

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Author(s):
Tramontina, Robson [1, 2] ; Brenelli, Livia Beatriz [1] ; Sousa, Amanda [1] ; Alves, Rafael [3] ; Zetty Arenas, Ana Maria [1, 4] ; Nascimento, Viviane Marcos [1] ; Rabelo, Sarita Candida [5] ; Freitas, Sindelia [1, 4, 6] ; Ruller, Roberto [7, 8] ; Squina, Fabio Marcio [9]
Total Authors: 10
Affiliation:
[1] CNPEM, Lab Nacl Biorrenovaveis LNBR, Campinas, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Programa Biociencias & Tecnol Prod Bioativos BTPB, Campinas, SP - Brazil
[3] Novozymes Amer Latina, Araucaria, PR - Brazil
[4] Univ Estadual Campinas UNICAMP, Fac Engn Alimentos, Programa Bioenergia, Campinas, SP - Brazil
[5] Univ Estadual Paulista Julio Mesquita Filho UNESP, Fac Ciencias Agron, Dept Bioproc & Biotecnol, Botucatu, SP - Brazil
[6] Univ Estadual Campinas UNICAMP, FEQ, Campinas, SP - Brazil
[7] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas IBILCE, Sao Jose Do Rio Preto, SP - Brazil
[8] Univ Fed Mato Grosso do Sul, Inst Biociencias, Lab Bioquim Geral & Microorganismos, Campo Grande, MS - Brazil
[9] Univ Sorocaba UNISO, Programa Proc Tecnol & Ambientais, Sorocaba, SP - Brazil
Total Affiliations: 9
Document type: Journal article
Source: Enzyme and Microbial Technology; v. 135, APR 2020.
Web of Science Citations: 0
Abstract

Bioproducts production using monomeric sugars derived from lignocellulosic biomass presents several challenges, such as to require a physicochemical pretreatment to improve its conversion yields. Hydrothermal lignocellulose pretreatment has several advantages and results in solid and liquid streams. The former is called hemicellulosic hydrolysate (HH), which contains inhibitory phenolic compounds and sugar degradation products that hinder microbial fermentation products from pentose sugars. Here, we developed and applied a novel enzyme process to detoxify HH. Initially, the design of experiments with different redox activities enzymes was carried out. The enzyme mixture containing the peroxidase (from Armoracia rusticana) together with superoxide dismutase (from Coptotermes gestroi) are the most effective to detoxify HH derived from sugarcane bagasse. Butanol fermentation by the bacteria Clostridium saccharoperbutylacetonicum and ethanol production by the yeast Scheffersomyces stipitis increased by 24.0 x and 2.4 x , respectively, relative to the untreated hemicellulosic hydrolysates. Detoxified HH was analyzed by chromatographic and spectrometric methods elucidating the mechanisms of phenolic compound modifications by enzymatic treatment. The enzyme mixture degraded and reduced the hydroxyphenyl- and feruloyl-derived units and polymerized the lignin fragments. This strategy uses biocatalysts under environmentally friendly conditions and could be applied in the fuel, food, and chemical industries. (AU)

FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via feluric acid to high value chemicals
Grantee:Fábio Márcio Squina
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 13/03061-0 - Characterization and antioxidant capacity of fractions of lignin extracted from sugarcane bagasse
Grantee:Lívia Beatriz Brenelli de Paiva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/15477-8 - Development of a low severity pretreatment process for the production of sugarcane straw oligosaccharides
Grantee:Lívia Beatriz Brenelli de Paiva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/26982-0 - Exploring novel strategies for depolymerization of plant cell-wall polysaccharides: from structure, function and rational design of glycosyl hydrolases to biological implications and potential biotechnological applications
Grantee:Mário Tyago Murakami
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/07926-4 - Novel integrative strategies through the aldoketo reductase from the termite Coptotermes gestroi for detoxification, saccharification and fermentation of lignocellulose aiming bioethanol production
Grantee:Robson Tramontina
Support type: Scholarships in Brazil - Doctorate