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Novel integrative strategies through the aldoketo reductase from the termite Coptotermes gestroi for detoxification, saccharification and fermentation of lignocellulose aiming bioethanol production

Grant number: 16/07926-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2016
Effective date (End): July 31, 2021
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Fábio Márcio Squina
Grantee:Robson Tramontina
Home Institution: Pró-Reitoria de Pós-Graduação, Pesquisa, Extensão e Inovação. Universidade de Sorocaba (UNISO). Sorocaba , SP, Brazil
Associated research grant:15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via feluric acid to high value chemicals, AP.BIOEN.TEM
Associated scholarship(s):17/23754-1 - Development of a synthetic route to produce coniferyl alcohol from ferulic acid using an Aldo-Keto Reductase from lower termite Coptotermes gestroi, BE.EP.DR

Abstract

The development of lignocellulosic biorefineries is the key to the sustainable bioethanol production. New technologies for the production of lignocellulolytic enzymes, protein engineering and the rational development of enzyme mixtures are of great importance in this area. In this context, the wood feeding insects, such as the termites, are biological systems considered as models for the study of plant biomass conversion with potential application in the biorefinery development. Preliminary results of our research group suggests that pro-oxidant, antioxidants and detoxifying enzymes (PADs) can be the advantage of termites which makes them one of the most efficient biological systems in the conversion of lignocellulose. Continuing the researches from the master degree, an aldoketo reductase from the termite Coptotermes gestroi (CgAKR-1) had proven its role in the insect digestomics. This enzyme was able to increase the efficiency of commercial cellulolytic cocktails in the hydrolysis of sugarcane bagasse. The enzymes also acted as a detoxication agent in the hemicellulose hydrolyzate from sugarcane bagasse. The detoxified hydrolizate was better fermented by xilose fermenting yeast after the enzymes treatment. In other words, the enzyme was proved to be a potential integrative enzyme in this chain. However, our studies revealed that the enzyme has no optimal thermal stability for industrial processes of sugar cane bagasse hydrolysis. Thus, the objectives of this PhD project focuses on the biotechnological applicability of an aldoketo reductase found in the termite Coptotermes gestroi (CgAKR-1). In order to achieve this goal we intend then: 1- improve the robustness of the enzyme against temperature compatible with the enzymatic hydrolysis of sugarcane bagasse; 2- heterologous production of CgAKR-1 in Aspergillus; 3- Develop saccharification process of sugarcane bagasse with cellulolytic cocktails supplemented with CgAKR-1; 4 Apply CgAKR-1 in the detoxification of fermentation inhibitors present in hemicellulose hydrolyzate, to increase efficiency in the fermentation of this source of pentoses.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TRAMONTINA, ROBSON; BRENELLI, LIVIA BEATRIZ; SOUSA, AMANDA; ALVES, RAFAEL; ZETTY ARENAS, ANA MARIA; NASCIMENTO, VIVIANE MARCOS; RABELO, SARITA CANDIDA; FREITAS, SINDELIA; RULLER, ROBERTO; SQUINA, FABIO MARCIO. Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms. Enzyme and Microbial Technology, v. 135, APR 2020. Web of Science Citations: 0.
DE SOUZA, WAGNER R.; MARTINS, POLYANA K.; FREEMAN, JACKIE; PELLNY, TILL K.; MICHAELSON, LOUISE V.; SAMPAIO, BRUNO L.; VINECKY, FELIPE; RIBEIRO, ANA P.; DA CUNHA, BARBARA A. D. B.; KOBAYASHI, ADILSON K.; DE OLIVEIRA, PATRICIA A.; CAMPANHA, RAQUEL B.; PACHECO, THALYTA F.; MARTARELLO, DANIELLY C. I.; MARCHIOSI, ROGERIO; FERRARESE-FILHO, OSVALDO; DOS SANTOS, WANDERLEY D.; TRAMONTINA, ROBSON; SQUINA, FABIO M.; CENTENO, DANILO C.; GASPAR, MARILIA; BRAGA, MARCIA R.; TINE, MARCO A. S.; RALPH, JOHN; MITCHELL, ROWAN A. C.; MOLINARI, HUGO B. C. Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility. NEW PHYTOLOGIST, v. 218, n. 1, p. 81-93, APR 2018. Web of Science Citations: 19.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.