The FBH family of bHLH transcription factors controls ACC synthase expression in sugarcane

Autor(es)

Alessio, Valter Miotto ^{[1, 2]} ; Cavacana, Natale ^[3] ; de Barros Dantas, Luiza Lane ^[3] ; Lee, Nayoung ^[1] ; Hotta, Carlos Takeshi ^[3] ; Imaizumi, Takato ^[1] ; Menossi, Marcelo ^[2]

Afiliação do(s) autor(es)

^[1] Univ Washington, Dept Biol, Seattle, WA 98195 - USA

^[2] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao Microbiol & Imunol, CP 6109, BR-13083862 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Experimental Botany; v. 69, n. 10, p. 2511-2525, APR 27 2018.
• Citações Web of Science: 3

Resumo

Ethylene is a phytohormone involved in the regulation of several aspects of plant development and in responses to biotic and abiotic stress. The effects of exogenous application of ethylene to sugarcane plants are well characterized as growth inhibition of immature internodes and stimulation of sucrose accumulation. However, the molecular network underlying the control of ethylene biosynthesis in sugarcane remains largely unknown. The chemical reaction catalyzed by 1-aminocyclopropane-1-carboxylic acid synthase (ACS) is an important rate-limiting step that regulates ethylene production in plants. In this work, using a yeast one-hybrid approach, we identified three basic helix-loophelix (bHLH) transcription factors, homologs of Arabidopsis FBH (FLOWERING BHLH), that bind to the promoter of ScACS2 (Sugarcane ACS2), a sugarcane type 3 ACS isozyme gene. Protein-protein interaction assays showed that sugarcane FBH1 (ScFBH1), ScFBH2, and ScFBH3 form homo-and heterodimers in the nucleus. Gene expression analysis revealed that ScFBHs and ScACS2 transcripts are more abundant in maturing internodes during afternoon and night. In addition, Arabidopsis functional analysis demonstrated that FBH controls ethylene production by regulating transcript levels of ACS7, a homolog of ScACS2. These results indicate that ScFBHs transcriptionally regulate ethylene biosynthesis in maturing internodes of sugarcane. (AU)

• Processo FAPESP: 13/15576-5 - Abordagens moleculares para estudar a relação entre etileno e maturação em cana-de-açúcar
• Beneficiário: Marcelo Menossi Teixeira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 15/06260-0 - Relógios biológicos específicos em órgãos e tecidos de gramíneas C4
• Beneficiário: Carlos Takeshi Hotta
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs