Do different probing depths exhibit striking differences in microbial profiles?

Autor(es)

Perez-Chaparro, Paula Juliana ^[1] ; McCulloch, John Anthony ^{[2, 3]} ; Mamizuka, Elsa Masae ^[2] ; Lima Moraes, Aline da Costa ^[4] ; Faveri, Marcelo ^[1] ; Figueiredo, Luciene Cristina ^[1] ; Duarte, Poliana Mendes ^[1] ; Feres, Magda ^[1]

Afiliação do(s) autor(es)

^[1] Univ Guarulhos, Dept Periodontol, Dent Res Div, Guarulhos - Brazil

^[2] Univ Sao Paulo, Dept Clin & Toxicol Anal, Fac Pharmaceut Sci, Sao Paulo - Brazil

^[3] NCI, Trinchieri Lab, Canc & Inflammat Program, NIH, Bethesda, MD 20892 - USA

^[4] Univ Estadual Campinas, Dept Plant Biol, Inst Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 45, n. 1, p. 26-37, JAN 2018.
• Citações Web of Science: 7

Resumo

AimTo perform a thorough characterization of the subgingival microbiota of shallow, moderate and deep sites in subjects with chronic periodontitis (ChP). Material and methodsSubgingival samples were collected from subjects with ChP (n=3/category of probing depth: 3, 4-6 and 7mm) and periodontal health (PH). Individual samples were submitted to 16S rDNA high- throughput sequencing and the analysis was made using mothur and R packages. ResultsNine subjects with ChP and seven with PH were included and 101 samples were evaluated. Thirteen phyla, 118 genera and 211 OTUs were detected. Taxa from Chloroflexi and Spirochaetes phyla were associated with initial stages of disease. Fretibacterium, Eubacterium{[}XI]{[}G-6], Desulfobulbus, Peptostreptococcaceae{[}XI]{[}G-1] and {[}G-3], Bacteroidetes{[}G-3], Bacteroidaceae{[}G-1] genera and Filifactor alocis, Fretibacterium fastidiosum, Johnsonella spHOT166, Peptostreptococcaceae{[}XIII]{[}G-1]HOT113, Porphyromonas endodontalis and Treponema sp. HOT258, which are not conventionally associated with disease, increased with the deepening of the pockets and/or were elevated in ChP; while Streptococcus, Corynebacterium and Bergeyella genera were associated with PH (p<.05). ConclusionStriking differences were observed between the microbiota of shallow and moderate/deep sites, but not between moderate and deep sites in ChP subjects. Differences between shallow sites in PH and ChP were also observed. The characterized microbiota included known oral microorganisms and newly identified periodontal taxa, some of them not-yet-cultured. (AU)

• Processo FAPESP: 13/12107-4 - Estudo da resistência heterogênea à vancomicina em Staphylococcus aureus através da genética comparativa de subpopulações de um isolado
• Beneficiário: John Anthony McCulloch
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/20915-0 - Análise metagenômica da microbiota periodontal subgengival em indivíduos com e sem doença periodontal
• Beneficiário: Paula Juliana Perez Chaparro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/23767-5 - Perfil microbiológico subgengival de indivíduos obesos e não-obesos com periodontite crônica
• Beneficiário: Suellen da Silva Maciel
• Modalidade de apoio: Bolsas no Brasil - Mestrado