Investigation of c-KIT and Ki67 expression in normal, preneoplastic and neoplastic canine prostate

Autor(es)

Fonseca-Alves, Carlos Eduardo ^[1] ; Kobayashi, Priscilla Emiko ^[1] ; Palmieri, Chiara ^[2] ; Laufer-Amorim, Renee ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, Rua Prof Dr Walter Mauricio Correa S-N, BR-18618681 Botucatu, SP - Brazil

^[2] Univ Queensland, Sch Vet Sci, Gatton Campus, Gatton, Qld - Australia

• Tipo de documento: Artigo Científico
• Fonte: BMC Veterinary Research; v. 13, DEC 6 2017.
• Citações Web of Science: 8

Resumo

Background: c-KIT expression has been related to bone metastasis in human prostate cancer, but whether c-KIT expression can be similarly classified in canine prostatic tissue is unknown. This study assessed c-KIT and Ki67 expression in canine prostate cancer (PC). c-KIT gene and protein expression and Ki67 expression were evaluated in forty-four canine prostatic tissues by immunohistochemistry, RT-qPCR and western blot. Additionally, we have investigated c-KIT protein expression by immunoblotting in two primary canine prostate cancer cell lines. Results: Eleven normal prostates, 12 proliferative inflammatory atrophy (PIA) prostates, 18 PC, 3 metastatic lesions and two prostate cancer cell cultures (PC1 and PC2) were analysed. The prostatic tissue exhibited varying degrees of membranous, cytoplasmic or membranous/cytoplasmic c-KIT staining. Four normal prostates, 4 PIA and 5 prostatic carcinomas showed positive c-KIT expression. No c-KIT immunoexpression was observed in metastases. Canine prostate cancer and PIA samples contained a higher number of Ki67-positive cells compared to normal samples. The median relative quantification (RQ) for c-KIT expression in normal, PIA and prostate cancer and metastatic samples were 0.6 (0.1-2.5), 0.7 (0.09-2.1), 0.7 (0.09-5.1) and 0.1 (0.07-0.6), respectively. A positive correlation between the number of Ki67-positive cells and c-KIT transcript levels was observed in prostate cancer samples. In the cell line, PC1 was negative for c-KIT protein expression, while PC2 was weakly positive. Conclusion: The present study identified a strong correlation between c-KIT expression and proliferative index, suggesting that c-KIT may influence cell proliferation. Therefore, c-KIT heterogeneous protein expression among the samples (five positive and thirteen negative prostate cancer samples) indicates a personalized approach for canine prostate cancer. (AU)

• Processo FAPESP: 12/16068-0 - Avaliação epigenética dos genes NKX3.1 e CDH1 e expressão do c-myc, NKX3.1 e caderina-E por imunoistoquímica em microarranjo de tecido (TMA) de lesões pré-neoplásicas e neoplásicas na próstata de cães
• Beneficiário: Renee Laufer Amorim
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/18426-1 - Avaliação epigenética dos genes NKX3.1 e CDH1 e expressão do C-MYC, NKX3.1 e caderina-E por imunoistoquímica em microarranjo de tecido (TMA) de lesões pré-neoplásicas e neoplásicas na próstata de cães
• Beneficiário: Carlos Eduardo Fonseca Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado