Salivary proteomics in lichen planus: A relationship with pathogenesis?

Autor(es)

Souza, M. M. ^[1] ; Florezi, G. P. ^[2] ; Nico, M. M. S. ^[1] ; de Paula, F. ^[2] ; Paula, F. M. ^[3] ; Lourenco, S. V. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Dermatol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Hosp Clin, Med Invest Lab, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 24, n. 5, p. 784-792, JUL 2018.
• Citações Web of Science: 2

Resumo

ObjectivesOral lichen planus is a chronic, T-cell-mediated, inflammatory disease that affects the oral cavity. The oral lichen planus pathogenesis is still unclear, however, the main evidence is that the mechanisms of activation of different T lymphocyte pathway induce apoptosis with an increase in Th1 and Th17 subtypes cells, triggered by the release of cytokines. This study analysed saliva proteomics to identify protein markers that might be involved in the pathogenesis and development of the disease. Material and MethodsProteins differentially expressed by oral lichen planus and healthy controls were screened using mass spectrometry; the proteins found in oral lichen planus were subjected to bioinformatics analysis, including gene ontology and string networks analysis. The multiplex analysis validation allowed the correlation between the proteins identified and the involved cytokines in Th17 response. ResultsOne hundred and eight proteins were identified in oral lichen planus, of which 17 proteins showed a high interaction between them and indicated an association with the disease. Expression of these proteins was correlated with the triggering of cytokines, more specifically the Th17 cells. ConclusionProteins, such as S100A8, S100A9, haptoglobin, can trigger cytokines and might be associated with a pathological function and antioxidant activities in oral lichen planus. (AU)

• Processo FAPESP: 14/11020-5 - O ENVOLVIMENTO DAS GLÂNDULAS SALIVARES NA DOENÇA DO ENXERTO CONTRA O HOSPEDEIRO: Estudo bioquímico e análise proteômica.
• Beneficiário: Silvia Vanessa Lourenço
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/11806-7 - Sialadenite na síndrome de Sjögren e no lúpus eritematoso: correlação clínico-patológica e análises proteômica e metabolômica da saliva
• Beneficiário: Silvia Vanessa Lourenço
• Modalidade de apoio: Auxílio à Pesquisa - Regular