Oral bacterial colonization on dental implants restored with titanium or zirconia abutments: 6-month follow-up

Autor(es)

de Freitas, Alice Ramos ^[1] ; de Oliveira Silva, Thalisson Saymo ^[1] ; Ribeiro, Ricardo Faria ^[1] ; de Albuquerque Junior, Rubens Ferreira ^[1] ; Pedrazzi, Vinicius ^[1] ; do Nascimento, Cassio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Dent Mat & Prosthodont, Fac Dent Ribeirao Preto, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 22, n. 6, p. 2335-2343, JUL 2018.
• Citações Web of Science: 6

Resumo

This investigation aimed to characterize in a 6-month follow-up the microbial profile of implants restored with either titanium or zirconia abutments at the genus or higher taxonomic levels. Twenty healthy individuals indicative for implant-retained single restorations were investigated. Half of participants were restored with titanium and half with zirconia abutments. Biofilm was collected from the implant-related sites after 1, 3, and 6 months of loading. The 16S rDNA genes were amplified and sequenced with Roche/454 platform. A total of 596 species were identified in 360 samples and grouped in 18 phyla and 104 genera. Titanium- or zirconia-related sites as well as teeth showed similar total numbers of operational taxonomic units (OTUs) colonizing surfaces over time. Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes, and Actinobacteria were the most prevalent phyla with significant differences between different surfaces and time point. Unclassified genera were found in lower levels (1.71% up to 9.57%) on titanium and zirconia samples when compared with teeth, with no significant differences. Titanium- and zirconia-related surfaces are promptly colonized by a bacterial community similar to those found in the remaining adjacent teeth. Results suggest a selective adhesion of different bacterial genotypes for either titanium or zirconia surfaces. Data also indicate a significant interaction between the relative effects taxa, time point, and sampling site. The present study disclosed a wider spectrum of microorganisms colonizing either titanium- or zirconia-related microbiomes in very early stage of implant colonization, revealing differences and suggesting a probably specific mechanism for selective bacterial adhesion. (AU)

• Processo FAPESP: 10/12830-0 - Avaliação da contaminação peri e intra-implantar de próteses cimentadas sobre conectores de titânio ou zircônia pelas técnicas DNA checkerboard e 16S rDNAbased PCR
• Beneficiário: Ricardo Faria Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22876-8 - Avaliação clínica e microbiológica da contaminação microbiana peri-implantar de próteses cimentadas sobre conectores de titânio ou zircônia
• Beneficiário: Cássio Do Nascimento
• Modalidade de apoio: Auxílio à Pesquisa - Regular