Hybrid Conjugates Formed between Gold Nanoparticles and an Amyloidogenic Diphenylalanine-Cysteine Peptide

Autor(es)

Pelin, Juliane N. B. D. ^[1] ; Gatto, Emanuela ^[2] ; Venanzi, Mariano ^[2] ; Cavalieri, Francesca ^{[2, 3]} ; Oliveira, Cristiano L. P. ^[4] ; Martinho, Herculano ^[1] ; Silva, Emerson R. ^[5] ; Aguilar, Andrea M. ^[6] ; Souza, Juliana S. ^[1] ; Alves, Wendel A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre - Brazil

^[2] Univ Roma Tor Vergata, Dept Chem Sci & Technol, Rome - Italy

^[3] Univ Melbourne, Dept Chem & Biomol Engn, Melbourne, Vic - Australia

^[4] Univ Sao Paulo, Inst Fis, BR-05314970 Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Dept Biofis, BR-04023062 Sao Paulo - Brazil

^[6] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRYSELECT; v. 3, n. 24, p. 6756-6765, JUN 29 2018.
• Citações Web of Science: 2

Resumo

We investigated the effect of gold nanoparticles (AuNPs) on the aggregation of a CFF (C=cysteine; F=phenylalanine) tripeptide (derived from A beta peptide) in aqueous medium. Special attention was dedicated to the role of AuNPs as inducers and inhibitors during nucleation kinetics and the structure of the resulting scaffolds was carefully investigated. At millimolar concentrations, the tripeptide was found to form beta-sheet structures organized into long filaments. Spectral signatures and topography of the filaments were studied by Raman spectroscopy and atomic force microscopy (AFM), revealing that conjugation to AuNPs not only stabilizes the system, but also inhibit or enhance amyloid-like features depending on the synthesis route used in the preparation of AuNPs. Sodium borohydride (NaBH4) mediated synthesis of AuNPs gave rise to a strong absorption peak close to 520nm, indicating that AuNPs were dispersed, independently of the peptide concentration added in the reaction. However, when the peptide/gold salt mixture was heated at 60 degrees C, AuNPs and AuNP-decorated filaments were both formed in solution and the fractions of which population were found to be dependent on the {[}HAuCl4]/{[}CFF] ratio, as illustrated by TEM images. In addition, the insertion of AuNPs at the surface of CFF nanostructures can promote electron transfer from the metallic nanoparticles to the CFF surface, creating an n-type semiconductor, and causing a peak shift of the phenylalanine absorption band. (AU)

• Processo FAPESP: 15/24018-1 - Conjugados polímero-peptídeo para formulação de hidrogéis e seu uso em detecção
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• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 17/02317-2 - Interfaces em materiais: propriedades eletrônicas, magnéticas, estruturais e de transporte
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• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/24409-3 - Peptídeos Penetrantes de Células para Transporte de DNA plasmídial e microRNA: da Estrutura Nanoscópica a Entrega de Genes.
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• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático