Porous polymer monolithic columns with gold nanoparticles as an intermediate ligand for the separation of proteins in reverse phase-ion exchange mixed mode

Autor(es)

Terborg, Lydia ^[1] ; Masini, Jorge C. ^[2] ; Lin, Michelle ^[3] ; Lipponen, Katriina ^[4] ; Riekolla, Marja-Liisa ^[4] ; Svec, Frantisek ^[1]

Afiliação do(s) autor(es)

^[1] EO Lawrence Berkeley Natl Lab, Mol Foundry, Berkeley, CA 94720 - USA

^[2] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, BR-05513970 Sao Paulo - Brazil

^[3] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 - USA

^[4] Univ Helsinki, Dept Chem, Analyt Chem Lab, FIN-00014 Helsinki - Finland

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ADVANCED RESEARCH; v. 6, n. 3, p. 441-448, MAY 2015.
• Citações Web of Science: 21

Resumo

A new approach has been developed for the preparation of mixed-mode stationary phases to separate proteins. The pore surface of monolithic poly(glycidyl methacrylate-co-ethylene dimethacrylate) capillary columns was functionalized with thiols and coated with gold nanoparticles. The final mixed mode surface chemistry was formed by attaching, in a single step, alkanethiols, mercaptoalkanoic acids, and their mixtures on the free surface of attached gold nanoparticles. Use of these mixtures allowed fine tuning of the hydrophobic/hydrophilic balance. The amount of attached gold nanoparticles according to thermal gravimetric analysis was 44.8 wt.%. This value together with results of frontal elution enabled calculation of surface coverage with the alkanethiol and mercaptoalkanoic acid ligands. Interestingly, alkanethiols coverage in a range of 4.46-4.51 molecules/nm(2) significantly exceeded that of mercaptoalkanoic acids with 2.39-2.45 molecules/nm(2). The mixed mode character of these monolithic stationary phases was for the first time demonstrated in the separations of proteins that could be achieved in the same column using gradient elution conditions typical of reverse phase (using gradient of acetonitrile in water) and ion exchange chromatographic modes (applying gradient of salt in water), respectively. (C) 2014 Production and hosting by Elsevier B.V. on behalf of Cairo University. (AU)

• Processo FAPESP: 12/14472-9 - Preparo, caracterização e utilização de colunas monolíticas capilares poliméricas mistas explorando interações entre nano partículas de ouro e tióis
• Beneficiário: Jorge Cesar Masini
• Modalidade de apoio: Bolsas no Exterior - Pesquisa