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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of auxochrome group in disperse dyes bearing azo groups as chromophore center in the biotransformation and molecular docking prediction by reductase enzyme: Implications and assessment for environmental toxicity of xenobiotics

Texto completo
Autor(es):
Franco, Jefferson Honorio [1] ; da Silva, Bianca F. [1] ; Dias, Elisangela Franciscon G. [2] ; de Castro, Alexandre A. [3] ; Ramalho, Teodorico C. [3] ; Zanoni, Maria Valnice Boldrin [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] State Univ Julio de Mesquita Filho UNESP, Inst Chem, Sao Paulo - Brazil
[2] Univ Campinas UNICAMP, Sch Technol, Sao Paulo - Brazil
[3] Fed Univ Lavras UFLA, Dept Chem, Lavras, Minas Gerais - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 160, p. 114-126, SEP 30 2018.
Citações Web of Science: 4
Resumo

Synthetic azo dyes have increasingly become a matter of great concern as a result of the genotoxic and mutagenic potential of the products derived from azo dye biotransformation. This work evaluates the manner in which reducing enzymes produced by Escherichia colt (E. cob) act on three disperse dyes bearing azo groups, namely Disperse Red 73 (DR 73), Disperse Red 78 (DR 78), and Disperse Red 167 (DR 167). UV-Vis spectrophotometry, high-performance liquid chromatography with diode array detector (HPLC-DAD), and liquid chromatography mass spectrometry (LC-MS/MS) were applied towards the identification of the main products. Seven days of incubation of the azo dyes with the tested enzymes yielded a completely bleached solution. 3-4-Aminophenyl-ethyl-amino-propanitrile was detected following the biotransformation of both DR 73 and DR 78. 4-Nitroaniline and 2-chloro-4-nitroaniline were detected upon the biotransformation of DR 73 and DR 78, respectively. The main products derived from the biotransformation of DR 167 were dimethyl 3,3'-3-acetamido-4-aminophenyl-azanedyl-dipropanoate and 2-chloro-4-nitroaniline. The results imply that DR 73 lost the CN-substituent during the biotransformation. Furthermore, theoretical calculations were also carried out aiming at evaluating the interaction and reactivity of these compounds with DNA. Taken together, the results indicate that DR 73, DR 78, and DR 167 pose health risks and serious threats to both human beings and the environment at large as their biotransformation produces harmful compounds such as amines, which have been widely condemned by the International Agency for Research on Cancer. (AU)

Processo FAPESP: 08/10449-7 - Avaliação da ocorrência, toxicidade/genotoxicidade e processos para degradação de corantes em efluentes e águas superficiais
Beneficiário:Maria Valnice Boldrin
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/18109-4 - Contribuição dos materiais nanoestruturados na eletroanálise de fármacos e corantes, na oxidação e redução fotoeletrocatalítica de poluentes orgânicos e inorgânicos e desinfecção de água
Beneficiário:Maria Valnice Boldrin
Linha de fomento: Auxílio à Pesquisa - Regular