Magnetically triggered release of amoxicillin from xanthan/Fe3O4/albumin patches

Autor(es)

Bueno, Pedro V. A. ^[1] ; Hilamatu, Karina C. P. ^[1] ; Carmona-Ribeiro, Ana M. ^[2] ; Petri, Denise F. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 115, p. 792-800, AUG 2018.
• Citações Web of Science: 3

Resumo

This work was motivated by the need of stimuli responsive drug carriers, which can be activated by low cost non-invasive stimuli such as external magnetic field (EMF). Thus, novel antimicrobial materials based on xanthan gum (XG), magnetic nanoparticles (MNP), bovine serum albumin (BSA) and amoxicillin (Amox) were designed in order to promote the release of Amox under magnetic stimuli. Firstly, surfaces with different functionalities were prepared by sequential deposition of thin layers on Si wafers and characterized by means of ellipsometry and atomic force microscopy. Amox adsorbed preferentially onto XG or BSA films. In solution, favorable interactions between Amox and BSA were evidenced by substantial changes in the BSA secondary structure, as revealed by circular dichroism. Patches of XG and XG/MNP/BSA were immersed in 2 g L-1 Amox, yielding 10 +/- 3 and 17 +/- 4 mu g/cm(3) Amox loading, respectively. The inclusion of 0.2 wt% Fe3O4 in the patches and their exposure to EMF enabled in vitro release of Amox, at pH 5.5 and 0.02 mol L-1 NaCI, following the quasi-Fickian behavior. Amox diffused from XG/MNP/BSA patches in agar medium containing Staphylococcus aureus and Escherichia coli, inhibiting their growth. The inhibition of E. coli growth was particularly efficient under EMF. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 16/08750-7 - Complexos híbridos de polieletrólitos e magnetita para liberação controlada de amoxicilina
• Beneficiário: Pedro Vinicius de Assis Bueno
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/25103-2 - Estímulos magnéticos em processos biotecnológicos
• Beneficiário: Denise Freitas Siqueira Petri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/07862-6 - Estímulos magnéticos em processos biotecnológicos
• Beneficiário: Karina Cassia Pandelo Hilamatu
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico