Molecular and Immnune Diagnosis: Further Testing for Human Strongyloidiasis

Autor(es)

Bosqui, Larissa R. ^[1] ; Marques, Priscilla D. ^[2] ; de Melo, Gessica B. ^[2] ; Goncalves-Pires, Maria do Rosario F. ^[3] ; Malta, Fernanda M. ^[2] ; Pavanelli, Wander R. ^[1] ; Conchon-Costa, Ivete ^[1] ; Costa-Cruz, Julia M. ^[3] ; Paula, Fabiana M. ^[2] ; Costa, Idessania N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Dept Ciencias Patol, CCB, Lab Parasitol Expt, Rodovia Celso Garcia Cid Campus Univ, BR-86051990 Londrina, PR - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas & Parasitarias, Hosp Clin, Ave Dr Arnaldo, 455 Cerqueira Cesar, BR-01246903 Sao Paulo, SP - Brazil

^[3] Univ Fed Uberlandia, Dept Parasitol, Inst Ciencias Biomed, Ave Para 1720, BR-38400902 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR DIAGNOSIS & THERAPY; v. 22, n. 4, p. 485-491, AUG 2018.
• Citações Web of Science: 2

Resumo

Detection of Strongyloides stercoralis larvae is particularly challenging because only a small number of larvae are released into the feces, regardless of infection stage. Our objective was to apply conventional polymerase chain reaction (PCR) to the detection of S. stercoralis DNA in feces samples to evaluate its performance in samples of patients with strongyloidiasis and compare results with those of immunodiagnosis. Stool, serum, and saliva samples were collected from each individual (n = 48) at the clinic hospital of the State University of Londrina, Brazil, for parasitological, immunological, and molecular tests. Stool samples were processed via parasitological methods. Serum samples were used for immunoglobulin G (IgG) detection and saliva samples for IgA detection by ELISA. For amplification by conventional PCR, two different primers were used: species specific (101 bp) and genus specific (392 bp). The results showed that 34 (97.1%) of the 35 copro-positive individuals for S. stercoralis were positive for serum IgG and 19 (54.3%) were positive for salivary IgA. Regarding molecular analysis, both primers (species and genus specific) demonstrated positivity in 100% of the samples, which was confirmed by sequencing the positive samples. Complementary examinations of the parasitological method demonstrated excellent results in the context of the diagnosis of strongyloidiasis, especially in asymptomatic patients with irregular larval release in the feces. (AU)

• Processo FAPESP: 13/03304-0 - Diagnóstico molecular da infecção por Strongyloides venezuelensis pela detecção específica de DNA em amostras de sangue de ratos experimentalmente infectados
• Beneficiário: Fabiana Martins de Paula
• Modalidade de apoio: Auxílio à Pesquisa - Regular