Hippocampal microRNA-mRNA regulatory network is affected by physical exercise

Autor(es)

Fernandes, Jansen ^[1] ; Vieira, Andre Schwambach ^[2] ; Kramer-Soares, Juliana Carlota ^[3] ; Da Silva, Eduardo Alves ^[1] ; Lee, Kil Sun ^[4] ; Lopes-Cendes, Iscia ^[5] ; Arida, Ricardo Mario ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Physiol, Sao Paulo, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Dept Psychobiol, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Dept Biochem, Sao Paulo, SP - Brazil

^[5] Univ Campinas UNICAMP, Sch Med Sci, Dept Med Genet, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS; v. 1862, n. 8, p. 1711-1720, AUG 2018.
• Citações Web of Science: 6

Resumo

Background: It is widely known that physical activity positively affects the overall health and brain function. Recently, microRNAs (miRNAs) have emerged as potential regulators of numerous biological processes within the brain. These molecules modulate gene expression post-transcriptionally by inducing mRNA degradation and inhibiting the translation of target mRNAs. Methods: To verify whether the procognitive effects of physical exercise are accompanied by changes in the activity of miRNA-mRNA network in the brain, differential expression analysis was performed in the hippo campus of control (CTL) and exercised (Ex) rats subjected to 4 weeks of treadmill exercise. Cognition was evaluated by a multiple trial inhibitory avoidance (MTIA) task and Illumina next-generation sequencing (NGS) was used for miRNA and mRNA profiling. Results: Exercise improved memory retention but not acquisition in the MTIA task. It was observed that 4 miRNAs and 54 mRNAs were significantly altered in the hippocampus of Ex2 (euthanized 2 h after the last exercise bout) group when compared to CTL group. Bioinformatic analysis showed an inverse correlation between 3 miRNAs and 6 target mRNAs. The miRNAs miR-129-1-3p and miR-144-5p were inversely correlated to the Igibp5 and Itm2a, respectively, and the miR-708-5p presented an inverse correlation with Cdkn1a, Per2, Rt1-A2. Conclusion: The exercise-induced memory improvements are accompanied by changes in hippocampal miRNA-mRNA regulatory network. (AU)

• Processo FAPESP: 13/13725-3 - Influência do Exercício Aeróbico Sobre a Expressão e a Distribuição de microRNAs na Formação Hipocampal de Ratos Adultos
• Beneficiário: Jansen Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50680-2 - Investigação multimodal da epileptogênese com ênfase na incorporação de novos modelos e novas ferramentas
• Beneficiário: Iscia Teresinha Lopes Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Temático