Tb II-I, a Fraction Isolated from Tityus bahiensis Scorpion Venom, Alters Cytokines': Level and Induces Seizures When Intrahippocampally Injected in Rats

Autor(es)

Neto, Emidio Beraldo ^[1] ; Mariano, Douglas O. C. ^[2] ; Freitas, Lucas A. ^[1] ; Dorce, Ana L. C. ^[1] ; Martins, Adriana N. ^[1] ; Pimenta, Daniel C. ^[2] ; Portaro, Fernanda C. V. ^[3] ; Cajado-Carvalho, Daniela ^[3] ; Dorce, Valquiria A. C. ^[1] ; Nencioni, Ana L. A. ^[1]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Pharmacol Lab, Ave Dr Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil

^[2] Butantan Inst, Biochem & Biophys Lab, Ave Dr Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil

^[3] Butantan Inst, Lab Immunochem, Ave Dr Vital Brazil 1500, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXINS; v. 10, n. 6 JUN 2018.
• Citações Web of Science: 3

Resumo

Scorpion venoms are composed of several substances with different pharmacological activities. Neurotoxins exert their effects by targeting ion channels resulting in toxic effects to mammals, insects and crustaceans. Tb II-I, a fraction isolated from Tityus bahiensis scorpion venom, was investigated for its ability to induce neurological and immune-inflammatory effects. Two putative -sodium channel toxins were identified in this fraction, Tb2 II and Tb 4, the latter having been completely sequenced by mass spectrometry. Male Wistar rats, stereotaxically implanted with intrahippocampal cannulas and electrodes, were injected with Tb II-I (2 mu g/2 mu L) via the intrahippocampal route. The behavior, electrographic activity and cellular integrity of the animals were analyzed and the intracerebral level of cytokines determined. Tb II-I injection induced seizures and damage in the hippocampus. These alterations were correlated with the changes in the level of the cytokines tumoral necrosis factor- (TNF-) and interleukin-6 (IL-6). Therefore, the binding of Tb II-I to its target in the central nervous system may induce inflammation resulting in neuropathological and behavioral alterations. (AU)

• Processo FAPESP: 16/10196-8 - Exposição de ratas Wistar lactantes ao veneno do escorpião Tityus bahiensis: efeitos na composição do leite e nos níveis de neurotransmissores e citocinas maternos e da prole.
• Beneficiário: Ana Leonor Abrahao Nencioni Soares
• Modalidade de apoio: Auxílio à Pesquisa - Regular