Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin

Autor(es)

Damiao Andrucioli, Marcela Cristina ^[1] ; Nakane Matsumoto, Mirian Aiko ^[1] ; Pereira Saraiva, Maria Conceicao ^[1] ; Feres, Magda ^[2] ; de Figueiredo, Luciene Cristina ^[2] ; Sorgi, Carlos Arterio ^[3] ; Faccioli, Lucia Helena ^[3] ; Bezerra da Silva, Raquel Assed ^[1] ; Bezerra da Silva, Lea Assed ^[1] ; Nelson-Filho, Paulo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Clin Infantil, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Guarulhos, Dept Periodontia, Div Pesquisa Odontol, Guarulhos, SP - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 26, 2018.
• Citações Web of Science: 1

Resumo

Abstract Objectives Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay. Material and Methods The 15 successful and 10 failed mini-implants (1.6 mm diameter × 7.0 or 9.0 mm long), placed in the maxilla and/or mandible, were obtained from 15 patients undergoing orthodontic treatment. Data were analyzed statistically by the Wilcoxon rank-sum test using the SAS software (a=0.05). Results All 40 microbial species were detected in both groups of mini-implants, with different frequencies. No differences were observed between the groups with respect to microbial complexes (blue, purple, yellow, green, orange, red and other species) and endotoxin quantification (p>0.05). Conclusion Neither microbial contamination nor endotoxin quantification was determinant for the early loss of stability of the mini-implants. (AU)

• Processo FAPESP: 11/23822-0 - Mini-implantes ortodônticos: avaliação microbiológica e quantificação de citocinas pró-inflamatórias, de endotoxina bacteriana e de marcadores da osteoclastogênese, em casos de sucesso e de insucesso
• Beneficiário: Paulo Nelson Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular