Incorporation of silver nanoparticles on Ti7.5Mo alloy surface containing TiO2 nanotubes arrays for promoting antibacterial coating - In vitro and in vivo study

Autor(es)

Rosifini Alves Clar, Ana Paula ^[1] ; Konatu, Reginaldo T. ^[1] ; do Amaral Escada, Ana Lucia ^[1] ; de Souza Nunes, Miriam Celi ^[2] ; Maurer-Morelli, Claudia Vianna ^[2] ; Dias-Netipanyj, Marcela Ferreira ^[3] ; Popat, Ketul C. ^[3] ; Mantovani, Diego ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Engn, Mat & Technol Dept, UNESP, Guaratingueta, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Fac Med Sci, Dept Med Genet, Campinas, SP - Brazil

^[3] Colorado State Univ, Dept Mech Engn, Sch Biomed Engn, Ft Collins, CO 80523 - USA

^[4] Laval Univ, Met & Mat Engn, Dept Min, Quebec City, PQ - Canada

• Tipo de documento: Artigo Científico
• Fonte: Applied Surface Science; v. 455, p. 780-788, OCT 15 2018.
• Citações Web of Science: 1

Resumo

Bulk and surface properties are very important for materials used in biomedical applications. The development of new surface treatments, such as antibacterial coatings can directly affect the response of the surface. The purpose of this study was the development of antibacterial coating on the Ti7.5Mo alloy surface combining TiO2 nanotubes with silver nanoparticles incorporation using polydopamine assisted immobilization technique. Surface characterization analysis showed that silver nanoparticles were successfully immobilized. The concentration established was bactericidal, i.e., no bacteria grew after incubation. In vivo results showed that silver incorporation on the surface containing TiO2 nanotubes did not cause altered locomotor activity in zebrafish. On the other hand, it affected the cell adhesion on the surface. Thus, these results confirm the hypothesis that silver nanoparticles were totally incorporated to polydopamine on the surface containing TiO2 nanotubes and acted as the main mechanism the death of the bacteria by contact. (AU)

• Processo FAPESP: 13/08200-9 - Liberação de medicamentos na superfície da liga Ti-7,5 Mo revestida com nanotubos de TiO2
• Beneficiário: Ana Lúcia Do Amaral Escada
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/15640-8 - Estudos funcionais de genes de inflamação e microRNAs relacionados às crises epilépticas no modelo do zebrafish
• Beneficiário: Cláudia Vianna Maurer Morelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular