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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Site-selective photoinduced cleavage and profiling of DNA by chiral semiconductor nanoparticles

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Autor(es):
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Sun, Maozhong [1, 2] ; Xu, Liguang [1, 2] ; Qu, Aihua [1, 2] ; Zhao, Peng [3] ; Hao, Tiantian [1, 2] ; Ma, Wei [1, 2] ; Hao, Changlong [1, 2] ; Wen, Xiaodong [3] ; Colombari, Felippe M. [4] ; de Moura, Andre F. [4] ; Kotov, Nicholas A. [5] ; Xu, Chuanlai [1, 2] ; Kuang, Hua [1, 2]
Número total de Autores: 13
Afiliação do(s) autor(es):
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu - Peoples R China
[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Jiangsu - Peoples R China
[3] Chinese Acad Sci, Inst Coal Chem, Taiyuan, Shanxi - Peoples R China
[4] Univ Fed Sao Carlos, Dept Chem, Sao Carlos, SP - Brazil
[5] Univ Michigan, Dept Chem Engn, Biointerfaces Inst, Ann Arbor, MI 48109 - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: NATURE CHEMISTRY; v. 10, n. 8, p. 821-830, AUG 2018.
Citações Web of Science: 33
Resumo

Gene editing is an important genetic engineering technique that enables gene manipulation at the molecular level. It mainly relies on engineered nucleases of biological origin, whose precise functions cannot be replicated in any currently known abiotic artificial material. Here, we show that chiral cysteine-modified CdTe nanoparticles can specifically recognize and, following photonic excitation, cut at the restriction site GAT'ATC ('indicates the cut site) in double-stranded DNA exceeding 90 base pairs, mimicking a restriction endonuclease. Although photoinduced reactive oxygen species are found to be responsible for the cleavage activity, the sequence selectivity arises from the affinity between cysteine and the conformation of the specific DNA sequence, as confirmed by quantum-chemical calculations. In addition, we demonstrate non-enzymatic sequence-specific DNA incision in living cells and in vivo using these CdTe nanoparticles, which may help in the design of abiotic materials for gene editing and other biological applications. (AU)

Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
Beneficiário:Elson Longo da Silva
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 12/15147-4 - Estudo computacional da termodinâmica de associação de sistemas auto-organizados
Beneficiário:André Farias de Moura
Linha de fomento: Auxílio à Pesquisa - Regular