Site-selective photoinduced cleavage and profiling of DNA by chiral semiconductor nanoparticles

Autor(es):Mostrar menos -

Sun, Maozhong ^{[1, 2]} ; Xu, Liguang ^{[1, 2]} ; Qu, Aihua ^{[1, 2]} ; Zhao, Peng ^[3] ; Hao, Tiantian ^{[1, 2]} ; Ma, Wei ^{[1, 2]} ; Hao, Changlong ^{[1, 2]} ; Wen, Xiaodong ^[3] ; Colombari, Felippe M. ^[4] ; de Moura, Andre F. ^[4] ; Kotov, Nicholas A. ^[5] ; Xu, Chuanlai ^{[1, 2]} ; Kuang, Hua ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu - Peoples R China

^[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Jiangsu - Peoples R China

^[3] Chinese Acad Sci, Inst Coal Chem, Taiyuan, Shanxi - Peoples R China

^[4] Univ Fed Sao Carlos, Dept Chem, Sao Carlos, SP - Brazil

^[5] Univ Michigan, Dept Chem Engn, Biointerfaces Inst, Ann Arbor, MI 48109 - USA

• Tipo de documento: Artigo Científico
• Fonte: NATURE CHEMISTRY; v. 10, n. 8, p. 821-830, AUG 2018.
• Citações Web of Science: 33

Resumo

Gene editing is an important genetic engineering technique that enables gene manipulation at the molecular level. It mainly relies on engineered nucleases of biological origin, whose precise functions cannot be replicated in any currently known abiotic artificial material. Here, we show that chiral cysteine-modified CdTe nanoparticles can specifically recognize and, following photonic excitation, cut at the restriction site GAT'ATC ('indicates the cut site) in double-stranded DNA exceeding 90 base pairs, mimicking a restriction endonuclease. Although photoinduced reactive oxygen species are found to be responsible for the cleavage activity, the sequence selectivity arises from the affinity between cysteine and the conformation of the specific DNA sequence, as confirmed by quantum-chemical calculations. In addition, we demonstrate non-enzymatic sequence-specific DNA incision in living cells and in vivo using these CdTe nanoparticles, which may help in the design of abiotic materials for gene editing and other biological applications. (AU)

• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/15147-4 - Estudo computacional da termodinâmica de associação de sistemas auto-organizados
• Beneficiário: André Farias de Moura
• Modalidade de apoio: Auxílio à Pesquisa - Regular