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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Site-selective photoinduced cleavage and profiling of DNA by chiral semiconductor nanoparticles

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Sun, Maozhong [1, 2] ; Xu, Liguang [1, 2] ; Qu, Aihua [1, 2] ; Zhao, Peng [3] ; Hao, Tiantian [1, 2] ; Ma, Wei [1, 2] ; Hao, Changlong [1, 2] ; Wen, Xiaodong [3] ; Colombari, Felippe M. [4] ; de Moura, Andre F. [4] ; Kotov, Nicholas A. [5] ; Xu, Chuanlai [1, 2] ; Kuang, Hua [1, 2]
Total Authors: 13
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu - Peoples R China
[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Jiangsu - Peoples R China
[3] Chinese Acad Sci, Inst Coal Chem, Taiyuan, Shanxi - Peoples R China
[4] Univ Fed Sao Carlos, Dept Chem, Sao Carlos, SP - Brazil
[5] Univ Michigan, Dept Chem Engn, Biointerfaces Inst, Ann Arbor, MI 48109 - USA
Total Affiliations: 5
Document type: Journal article
Source: NATURE CHEMISTRY; v. 10, n. 8, p. 821-830, AUG 2018.
Web of Science Citations: 23

Gene editing is an important genetic engineering technique that enables gene manipulation at the molecular level. It mainly relies on engineered nucleases of biological origin, whose precise functions cannot be replicated in any currently known abiotic artificial material. Here, we show that chiral cysteine-modified CdTe nanoparticles can specifically recognize and, following photonic excitation, cut at the restriction site GAT'ATC ('indicates the cut site) in double-stranded DNA exceeding 90 base pairs, mimicking a restriction endonuclease. Although photoinduced reactive oxygen species are found to be responsible for the cleavage activity, the sequence selectivity arises from the affinity between cysteine and the conformation of the specific DNA sequence, as confirmed by quantum-chemical calculations. In addition, we demonstrate non-enzymatic sequence-specific DNA incision in living cells and in vivo using these CdTe nanoparticles, which may help in the design of abiotic materials for gene editing and other biological applications. (AU)

FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
Grantee:Elson Longo da Silva
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/15147-4 - Computational study of the association thermodynamics of self­Assembled systems
Grantee:André Farias de Moura
Support type: Regular Research Grants