Red disperse dyes (DR 60, DR 73 and DR 78) at environmentally realistic concentrations impact biochemical profile of early life stages of zebrafish (Danio rerio)

Autor(es)

Meireles, Gabriela ^{[1, 2, 3]} ; Daam, Michiel A. ^[4] ; Madeira Sanches, Ana Leticia ^{[2, 3, 5]} ; Zanoni, Maria V. B. ^{[6, 7]} ; Soares, Amadeu M. V. M. ^{[2, 3]} ; Gravato, Carlos ^{[8, 9]} ; de Oliveira, Danielle P. ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Aveiro, Dept Biol, P-3810193 Aveiro - Portugal

^[3] Univ Aveiro, CESAM, P-3810193 Aveiro - Portugal

^[4] Univ Nova Lisboa, Fac Sci & Technol, Dept Environm Sci & Engn, CENSE, P-2829516 Quinta Da Torre, Caparica - Portugal

^[5] Univ Sao Paulo, Sao Carlos Engn Sch, NEEA CRHEA SHS, BR-13560970 Sao Carlos, SP - Brazil

^[6] Conselho Nacl Desenvolvimento Cient & Tecnol CNPq, Natl Inst Sci & Technol Detect Toxicol Evaluat &, Brasilia, DF - Brazil

^[7] State Univ Julio de Mesquita Filho UNESP, Inst Chem, BR-14800900 Sao Paulo - Brazil

^[8] Univ Lisbon, Fac Sci, P-1749016 Lisbon - Portugal

^[9] Univ Lisbon, CESAM, P-1749016 Lisbon - Portugal

• Tipo de documento: Artigo Científico
• Fonte: Chemico-Biological Interactions; v. 292, p. 94-100, AUG 25 2018.
• Citações Web of Science: 2

Resumo

Dyes have been used for more than twenty thousand years in textile, pharmaceutical, food, cosmetic, and photographic industries, among others. Despite their importance in these applications, dyes can be toxic and resistant to many degradation processes used in wastewater treatment plants. Although a large number of dyes have been released in the environments in high amounts, studies into the environmental toxicity of these substances are still scarce. The aim of this study was to evaluate the potential toxic effects of textile dyes Disperse Red 60, Disperse Red 73 and Disperse Red 78 in zebrafish early life stages. To this end, biochemical biomarkers were selected to evaluate non-enzimatic antioxidant (Total Glutathione), antioxidant enzymes (Glutathione S-transferase and Catalase), oxidative stress (lipid peroxidation), neurotransmission (acetylcholinesterase) and energetic metabolism parameters (energy available and energy consumed) after 96 h exposure to these dyes. Our results demonstrated that these disperse dyes induce biochemical alterations in zebrafish embryos at environmental realistic concentrations and that the discharge of these disperse dyes into water bodies should be carefully evaluated. The selected biomarkers were sensitive as early-warning endpoints of disperse dyes toxicity on zebrafish embryos. Implications for risk assessment and indications for future research are discussed. (AU)

• Processo FAPESP: 17/05543-3 - Estudo comparativo dos efeitos tóxicos gerados em embriões e adultos de Danio rerio expostos ao inseticida abamectina e ao fungicida difenoconazol
• Beneficiário: Ana Letícia Madeira Sanches
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/14621-0 - Análise do efeito ambiental dos praguicidas kraft e score e de seus princípios ativos abamectina e difenoconazol em águas superficiais tropicais por meio de biomarcadores
• Beneficiário: Ana Letícia Madeira Sanches
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/03281-9 - Análises de alta performance (high-throughput screening) para avaliação da toxicidade neurocomportamental e no desenvolvimento induzidas após a exposição de embriões de zebrafish a contaminantes emergentes
• Beneficiário: Danielle Palma de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular