Genetic Diversity of Bartonella spp. in Wild Mammals and Ectoparasites in Brazilian Pantanal

Autor(es)

Marques de Sousa, Keyla Carstens ^[1] ; do Amaral, Renan Bressianini ^[1] ; Herrera, Heitor Miraglia ^[2] ; Santos, Filipe Martins ^[2] ; Macedo, Gabriel Carvalho ^[2] ; Estrela de Andrade Pinto, Pedro Cordeiro ^[3] ; Barros-Battesti, Darci Moraes ^[1] ; Machado, Rosangela Zacarias ^[1] ; Andre, Marcos Rogerio ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil

^[2] Univ Catolica Dom Bosco, Campo Grande, MS - Brazil

^[3] Univ Fed Paraiba, Lab Ecol Anim, Rio Tinto, PB - Brazil

^[4] Univ Estadual Paulista, Fac Ciencias Agr & Vet Julio Mesquita Filho, Dept Patol Vet, Lab Imunoparasitol, Campus Jaboticabal, BR-14884900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROBIAL ECOLOGY; v. 76, n. 2, p. 544-554, AUG 2018.
• Citações Web of Science: 5

Resumo

The present work aimed to investigate the genetic diversity of Bartonella in mammals and ectoparasites in Pantanal wetland, Brazil. For this purpose, 31 Nasua nasua, 78 Cerdocyon thous, 7 Leopardus pardalis, 110 wild rodents, 30 marsupials, and 42 dogs were sampled. DNA samples were submitted to a quantitative real-time PCR assay (qPCR). Positive samples in qPCR were submitted to conventional PCR assays targeting other five protein-coding genes. Thirty-five wild rodents and three Polygenis (P.) bohlsi bohlsi flea pools showed positive results in qPCR for Bartonella spp. Thirty-seven out of 38 positive samples in qPCR were also positive in cPCR assays based on ftsZ gene, nine in nuoG-cPCR, and six in gltA-cPCR. Concatenated phylogenetic analyses showed that two main genotypes circulate in rodents and ectoparasites in the studied region. While one of them was closely related to Bartonella spp. previously detected in Cricetidae rodents from North America and Brazil, the other one was related to Bartonella alsatica, Bartonella pachyuromydis, Bartonella birtlesii, Bartonella acomydis, Bartonella silvatica, and Bartonella callosciuri. These results showed that at least two Bartonella genotypes circulate among wild rodents. Additionally, the present study suggests that Polygenis (P.) bohlsi bohlsi fleas could act as possible Bartonella vectors among rodents in Pantanal wetland, Brazil. (AU)

• Processo FAPESP: 15/03262-1 - Detecção e caracterização molecular de Bartonella spp. em roedores e marsupiais selvagens no Pantanal Sul-mato grossense
• Beneficiário: Renan Bressianini do Amaral
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/14896-1 - Detecção e caracterização molecular de agentes das famílias Anaplasmataceae e Bartonellaceae em animais selvagens e ectoparasitas no Brasil
• Beneficiário: Marcos Rogério André
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/13186-5 - Detecção sorológica e caracterização molecular de agentes transmitidos por artrópodes em animais selvagens e domésticos na região do Pantanal Sul Matogrossense
• Beneficiário: Keyla Carstens Marques de Sousa
• Modalidade de apoio: Bolsas no Brasil - Doutorado