Investigation in vivo of Enterococcus faecalis in endodontic retreatment by phenotypic and genotypic methods

Autor(es)

Endo, Marcos Sergio ^[1] ; Correa Signoretti, Fernanda Graziela ^[1] ; Kitayama, Vivian Sayuri ^[2] ; Salustiano Marinho, Ariane Cassia ^[1] ; Martinho, Frederico Canato ^[3] ; de Almeida Gomes, Brenda Paula F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Odontol Restauradora, Div Endodontia, Fac Odontol Piracicaba, Av Limeira 901, BR-13414903 Sao Paulo - Brazil

^[2] Univ Estadual Maringa, Dept Odontol, Maringa, Parana - Brazil

^[3] Univ Estadual Sao Paulo, Dept Odontol Restauradora, Div Endodontia, Fac Odontol Sao Jose dos Campos, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACTA SCIENTIARUM-HEALTH SCIENCES; v. 37, n. 1, p. 95-103, JAN-JUN 2015.
• Citações Web of Science: 3

Resumo

This study aimed to investigate the prevalence of E. faecalis in root-filled canals using culture and molecular approaches. It was evaluated the antimicrobial susceptibility to different antibiotics and the virulence factors of E. faecalis isolates. Microbial samples were taken from thirty root-filled canals. Culture methods and 16S rDNA assay were used to identify E. faecalis. The antimicrobial susceptibility of E. faecalis was determined by MIC values using the E test. Cultivable strains of E. faecalis were investigated for virulence factors by PCR technique. E. faecalis were detected by culture (7/30), traditional PCR assay (13/30) and nested PCR (23/30). Both PCR were significantly more effective than culture in detecting E. faecalis (p < 0.05). All tested E. faecalis were highly sensitive to amoxicillin. Some strains of E. faecalis were resistant to antibiotics such as rifampicin (4/12), erythromycin (3/12) and azythromycin (8/12). The genes efaA and ace were detected in all isolates. The other virulence genes were found in 91.6 (gelE), 83.3 (asa), 25 (esp) and 16.6% (cylA). Strains of E. faecalis isolated from root-filled canals showed virulence factors related to adherence. They also showed resistance to some antibiotics commonly used in dentistry. (AU)

• Processo FAPESP: 11/50051-5 - Avaliacao in vivo do efeito do preparo quimico-mecanico sobre microrganismos e endotoxinas em infeccoes endodonticas persistentes e suscetibilidade antimicrobiana dos enterococcus spp. isolados.
• Beneficiário: Marcos Sergio Endo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50510-0 - Avaliação in vivo do efeito do preparo químico-mecânico sobre microrganismos e endotoxinas em infecções endodônticas persistentes e suscetibilidade antimicrobiana dos Enterococcus spp. isolados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular