Influence of chemical and physical conditions in selection of Gluconacetobacter hansenii ATCC 23769 strains with high capacity to produce bacterial cellulose for application as sustained antimicrobial drug-release supports

Autor(es)

Lazarini, S. C. ^[1] ; Yamada, C. ^[1] ; Barud, H. S. ^[1] ; Trovatti, E. ^[1] ; Corbi, P. P. ^[2] ; Lustri, W. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Araraquara, Dept Biol & Hlth Sci, UNIARA, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Inst Chem, UNICAMP, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Microbiology; v. 125, n. 3, p. 777-791, SEP 2018.
• Citações Web of Science: 2

Resumo

AimsObtain varieties of Gluconacetobacter hansenii from original strain ATCC 23729 with greater efficiency to produce bacterial cellulose (BC) membrane with better dry mass yield for application as support of sustained antimicrobials' drug release. Methods and resultsApplication of different chemical and physical conditions (pH, temperature and UV light exposure) to obtain different G. hansenii varieties with high capacity to produce BC membranes. Characterization of the G. hansenii variants was performed by scanning electron microscopy (SEM) and optical microscopy of the colony-forming units. BC membrane produced was characterized by SEM, infrared spectroscopy and X-ray diffraction. The BC produced by variants isolated after incubation at 35 degrees C showed elevated dry mass yield and high capacity of retention and sustained release of ceftriaxone antibiotic with the produced BC by original G. hansenii ATCC 23769 strain subjected to incubation at 28 degrees C and with commercial BC. ConclusionThe application of different chemical and physical conditions constitutes an important method to obtain varieties of micro-organisms with dissimilar metabolism advantageous in relation to the original strain in the BC production. Significance and Impact of the StudyThese results demonstrate the importance of invivo studies for the application, in medicine, of BC membranes as support for antimicrobial-sustained release for the skin wound treatment. (AU)

• Processo FAPESP: 15/09833-0 - Dispositivos de liberação sustentada baseados em biocelulose/complexos metálicos com ligantes bioativos
• Beneficiário: Wilton Rogério Lustri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/25114-4 - Preparação de novos complexos de Ag(I), Cu(II), Pd(II) e Pt(II) à base de sulfonamidas e derivados: estudos de atividade antibacteriana e perspectivas de aplicação em lesões de pele criticamente colonizadas.
• Beneficiário: Pedro Paulo Corbi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/17278-0 - Produção de celulose bacteriana utilizando diferentes substratos para uso em sistema de liberação sustentada de fármacos antibacterianos
• Beneficiário: Caroline Yamada
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica