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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of chemical and physical conditions in selection of Gluconacetobacter hansenii ATCC 23769 strains with high capacity to produce bacterial cellulose for application as sustained antimicrobial drug-release supports

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Lazarini, S. C. [1] ; Yamada, C. [1] ; Barud, H. S. [1] ; Trovatti, E. [1] ; Corbi, P. P. [2] ; Lustri, W. R. [1]
Total Authors: 6
[1] Univ Araraquara, Dept Biol & Hlth Sci, UNIARA, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Inst Chem, UNICAMP, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Applied Microbiology; v. 125, n. 3, p. 777-791, SEP 2018.
Web of Science Citations: 1

AimsObtain varieties of Gluconacetobacter hansenii from original strain ATCC 23729 with greater efficiency to produce bacterial cellulose (BC) membrane with better dry mass yield for application as support of sustained antimicrobials' drug release. Methods and resultsApplication of different chemical and physical conditions (pH, temperature and UV light exposure) to obtain different G. hansenii varieties with high capacity to produce BC membranes. Characterization of the G. hansenii variants was performed by scanning electron microscopy (SEM) and optical microscopy of the colony-forming units. BC membrane produced was characterized by SEM, infrared spectroscopy and X-ray diffraction. The BC produced by variants isolated after incubation at 35 degrees C showed elevated dry mass yield and high capacity of retention and sustained release of ceftriaxone antibiotic with the produced BC by original G. hansenii ATCC 23769 strain subjected to incubation at 28 degrees C and with commercial BC. ConclusionThe application of different chemical and physical conditions constitutes an important method to obtain varieties of micro-organisms with dissimilar metabolism advantageous in relation to the original strain in the BC production. Significance and Impact of the StudyThese results demonstrate the importance of invivo studies for the application, in medicine, of BC membranes as support for antimicrobial-sustained release for the skin wound treatment. (AU)

FAPESP's process: 15/25114-4 - Synthesis of new ag(i), Cu(II), Pd(II) and Pt(II) complexes based on sulfonamides and derivatives: antibacterial activities and application prospects on skin critically colonized wounds
Grantee:Pedro Paulo Corbi
Support type: Regular Research Grants
FAPESP's process: 16/17278-0 - Bacterial cellulose production using different substrates for use in sustained release system for antibacterial agents
Grantee:Caroline Yamada
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/09833-0 - Sustainable delivery system based on biocellulose/metallic complexes containing bioactive ligands
Grantee:Wilton Rogério Lustri
Support type: Regular Research Grants