Glycaemic status affects the subgingival microbiome of diabetic patients

Autor(es)

Longo, Priscila L. ^[1] ; Dabdoub, Shareef ^[2] ; Kumar, Purnima ^[2] ; Artese, Hilana P. C. ^[3] ; Dib, Sergio A. ^[4] ; Romito, Giuseppe A. ^[3] ; Alves Mayer, Marcia Pinto ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Av Prof Lineu Prestes 1374, BR-05508900 Sao Paulo, SP - Brazil

^[2] Ohio State Univ, Coll Dent, Div Periodontol, Columbus, OH 43210 - USA

^[3] Univ Sao Paulo, Sch Dent, Dept Stomatol, Div Periodont, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Sch Med, Dept Endocrinol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 45, n. 8, p. 932-940, AUG 2018.
• Citações Web of Science: 4

Resumo

AimPeriodontitis iscorrelated with type 2 diabetes mellitus (T2DM), but little is known about glycaemic status effect on subgingival microbiota associated withperiodontitis. This study evaluated if periodontal microbiome of T2DM patients is affected by glycaemic status. Materials and methodsTwenty-one T2DM non-smoking patients with chronic periodontitis and body mass index 40kg/m(2) were allocated into two groups according to systemic glycaemic status: inadequate (DMI- HbA1c8%) and adequate (DMA- HbA1c <7.8%). Subgingival biofilm was collected from sites with moderate (PD=4-6mm) and severe disease (PD7mm) in two quadrants. The V5-V6 hypervariable region of the 16SrRNA was sequenced using the GS-FLX-454 Titanium platform. Sequences were compared with HOMD database using QIIME and PhyloToAST pipelines. Statistical comparisons were made using two-sample t-tests. ResultsDMA microbiome presented higher diversity than DMI. Inadequate glycaemic control favoured fermenting species, especially those associated with propionate/succinate production, whereas those forming butyrate/pyruvate was decreased in DMI. Higher abundances of anginosus group and Streptococcus agalactiae in DMI may indicate that subgingival sites can be reservoir of potentially invasive pathogens. Altered subgingival microbiome in DMI may represent an additional challenge in the periodontal treatment of these patients and in the prevention of more invasive infections. ConclusionGlycaemic status in T2DM patients seems to modulate subgingival biofilm composition. (AU)

• Processo FAPESP: 13/20537-9 - Análise dos dados gerados por sequenciamento de alta performance da região 16SrRNA em amostras de biofilme subgengival de pacientes diabéticos
• Beneficiário: Priscila Larcher Longo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 11/10057-4 - Análise da diversidade da microbiota subgengival e perfil de citocinas séricas e de fluido gengival relacionada ao controle glicêmico de indivíduos com diabetes mellitus tipo II
• Beneficiário: Priscila Larcher Longo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/18618-5 - Análise da diversidade da microbiota subgengival e perfil de citocinas séricas e de fluido gengival relacionados ao controle glicêmico de indivíduos com Diabetes Mellitus tipo II
• Beneficiário: Marcia Pinto Alves Mayer
• Modalidade de apoio: Auxílio à Pesquisa - Regular