| Texto completo | |
| Autor(es): |
Santos, Elisa G.
[1]
;
Silva, Maraisa A.
[2]
;
Amorim, Renata P.
[1]
;
Giordano, Leticia de Souza
[2]
;
Silva, Dayana de Sales
[3]
;
Rasmussen, Lucas T.
[1, 2, 3]
;
Peruquetti, Rita L.
[1, 2, 3]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Sagrado Coracao Univ USC, Off Associate Dean Grad Studies & Res, BR-17011160 Bauru, SP - Brazil
[2] Sagrado Coracao Univ USC, Sch Hlth Sci, BR-17011160 Bauru, SP - Brazil
[3] Sagrado Coracao Univ USC, Mol Biol & Cytogenet Lab, BR-17011160 Bauru, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Experimental Biology and Medicine; v. 243, n. 11, p. 917-925, JUL 2018. |
| Citações Web of Science: | 0 |
| Resumo | |
The chromatoid body is a cytoplasmic male germ cell structure that plays a role in the regulation of mRNA transcription during spermatogenesis. A proteomic analysis of this structure has identified the presence of its classic molecular markers (MVH and MIWI), as well as a significant number of transient proteins. Circadian locomotor output cycles protein kaput (CLOCK) and brain and muscle ARNT-like 1 (BMAL1), which are molecular components of the circadian clock, are likely located in the chromatoid body in a transient fashion. This study sought to determine whether aging produces morphological changes in the chromatoid bodies of round spermatids similar to those previously observed in BMAL1 knockout mice. A sample of 30 male mice was divided into three groups: juvenile mice (45 days old), adult mice (120 days old), and old mice (+180 days old). Aging was confirmed by viability and sperm count analyses and testosterone dosage. Squash slides prepared with fragments of seminiferous tubules were immunostained for MVH, MIWI, BMAL1, and CLOCK detection. In juvenile and adult specimens, single round chromatoid bodies were observed using MVH/BMAL1 and MIWI/CLOCK immunostaining. In old specimens, many chromatoid bodies displayed changes in number and morphology, as well as an increase in the interactions between MVH and BMAL1; MIWI and CLOCK. Changes in chromatoid body morphology increased interactions between the proteins analyzed herein, and decreased amounts of these proteins in seminiferous tubules of older mice may indicate that aging influences the assembly and physiology of chromatoid bodies, which may, in turn, affect fertility. (AU) | |
| Processo FAPESP: | 16/04580-0 - Desvendando o papel de proteínas transitórias na composição molecular de chromatoid bodies de mamíferos: Fibrilarina (proteína nucleolar) e CLOCK/BMAL1 (controle ciclo circadiano) |
| Beneficiário: | Rita Luiza Peruquetti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/01554-3 - Desvendando o papel de proteínas transitórias na composição molecular de chromatoid bodies de mamíferos: fibrilarina (proteína nucleolar) e CLOCK/ bmal1 (controle ciclo circadiano) |
| Beneficiário: | Letícia de Souza Giordano |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 12/22009-7 - Descrição da distribuição da proteína nucleolar fibrilarina no epitélio seminífero de mamíferos e caracterização do seu papel na manutenção da fisiologia de chromatoid bodies |
| Beneficiário: | Rita Luiza Peruquetti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |