Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Aging and chromatoid body assembly: Are these two physiological events linked?

Full text
Author(s):
Santos, Elisa G. [1] ; Silva, Maraisa A. [2] ; Amorim, Renata P. [1] ; Giordano, Leticia de Souza [2] ; Silva, Dayana de Sales [3] ; Rasmussen, Lucas T. [1, 2, 3] ; Peruquetti, Rita L. [1, 2, 3]
Total Authors: 7
Affiliation:
[1] Sagrado Coracao Univ USC, Off Associate Dean Grad Studies & Res, BR-17011160 Bauru, SP - Brazil
[2] Sagrado Coracao Univ USC, Sch Hlth Sci, BR-17011160 Bauru, SP - Brazil
[3] Sagrado Coracao Univ USC, Mol Biol & Cytogenet Lab, BR-17011160 Bauru, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Experimental Biology and Medicine; v. 243, n. 11, p. 917-925, JUL 2018.
Web of Science Citations: 0
Abstract

The chromatoid body is a cytoplasmic male germ cell structure that plays a role in the regulation of mRNA transcription during spermatogenesis. A proteomic analysis of this structure has identified the presence of its classic molecular markers (MVH and MIWI), as well as a significant number of transient proteins. Circadian locomotor output cycles protein kaput (CLOCK) and brain and muscle ARNT-like 1 (BMAL1), which are molecular components of the circadian clock, are likely located in the chromatoid body in a transient fashion. This study sought to determine whether aging produces morphological changes in the chromatoid bodies of round spermatids similar to those previously observed in BMAL1 knockout mice. A sample of 30 male mice was divided into three groups: juvenile mice (45 days old), adult mice (120 days old), and old mice (+180 days old). Aging was confirmed by viability and sperm count analyses and testosterone dosage. Squash slides prepared with fragments of seminiferous tubules were immunostained for MVH, MIWI, BMAL1, and CLOCK detection. In juvenile and adult specimens, single round chromatoid bodies were observed using MVH/BMAL1 and MIWI/CLOCK immunostaining. In old specimens, many chromatoid bodies displayed changes in number and morphology, as well as an increase in the interactions between MVH and BMAL1; MIWI and CLOCK. Changes in chromatoid body morphology increased interactions between the proteins analyzed herein, and decreased amounts of these proteins in seminiferous tubules of older mice may indicate that aging influences the assembly and physiology of chromatoid bodies, which may, in turn, affect fertility. (AU)

FAPESP's process: 16/04580-0 - Decifring the role of transient proteins in the chromatoid bodies molecular composition: Fibrillarin (nucleolar component) and CLOCK/BMAL1 (circadian proteins).
Grantee:Rita Luiza Peruquetti
Support Opportunities: Regular Research Grants
FAPESP's process: 18/01554-3 - Decifring the role of transient proteins in the chromatoid bodies molecular composition: fibrillarin (nucleolar component) and CLOCK/BMAL1 (circadian proteins)
Grantee:Letícia de Souza Giordano
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
FAPESP's process: 12/22009-7 - Distribution of nucleolar protein fibrillarin in the seminiferous epithelium of mammals and its role in the chromatoid bodies physiology
Grantee:Rita Luiza Peruquetti
Support Opportunities: Regular Research Grants