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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A gene-rich fraction analysis of the Passiflora edulis genome reveals highly conserved microsyntenic regions with two related Malpighiales species

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Autor(es):
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Munhoz, Carla Freitas [1] ; Costa, Zirlane Portugal [1] ; Cauz-Santos, Luiz Augusto [1] ; Egoavil Reategui, Alina Carmen [1] ; Rodde, Nathalie [2] ; Cauet, Stephane [2] ; Dornelas, Marcelo Carnier [3] ; Leroy, Philippe [4] ; Varani, Alessandro de Mello [5] ; Berges, Helene [2] ; Carneiro Vieira, Maria Lucia [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba - Brazil
[2] INRA, Ctr Natl Ressources Genom Vegetales, F-31326 Castanet Tolosan - France
[3] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083862 Campinas, SP - Brazil
[4] UCA, INRA, UMR 1095, GDEC, F-63000 Clermont Ferrand - France
[5] Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 8, AUG 29 2018.
Citações Web of Science: 3
Resumo

Passiflora edulis is the most widely cultivated species of passionflowers, cropped mainly for industrialized juice production and fresh fruit consumption. Despite its commercial importance, little is known about the genome structure of P. edulis. To fill in this gap in our knowledge, a genomic library was built, and now completely sequenced over 100 large-inserts. Sequencing data were assembled from long sequence reads, and structural sequence annotation resulted in the prediction of about 1,900 genes, providing data for subsequent functional analysis. The richness of repetitive elements was also evaluated. Microsyntenic regions of P. edulis common to Populus trichocarpa and Manihot esculenta, two related Malpighiales species with available fully sequenced genomes were examined. Overall, gene order was well conserved, with some disruptions of collinearity identified as rearrangements, such as inversion and translocation events. The microsynteny level observed between the P. edulis sequences and the compared genomes is surprising, given the long divergence time that separates them from the common ancestor. P. edulis gene-rich segments are more compact than those of the other two species, even though its genome is much larger. This study provides a first accurate gene set for P. edulis, opening the way for new studies on the evolutionary issues in Malpighiales genomes. (AU)

Processo FAPESP: 17/04216-9 - Genomas cloroplastidiais de passifloras: estrutura, inferências evolutivas e transferência de genes para o núcleo
Beneficiário:Luiz Augusto Cauz dos Santos
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 14/25215-2 - Descobrindo o genoma de Passiflora: sequenciamento de BAC-ends e de BACs candidatos
Beneficiário:Maria Lúcia Carneiro Vieira
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/11196-3 - Biblioteca genômica de Passiflora edulis f. flavicarpa: exploração por sequenciamento de BAC-ends e sequenciamento de BACs candidatos
Beneficiário:Carla de Freitas Munhoz
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado