NF-kappa B and Angiogenesis Inhibitors from the Aerial Parts of Chresta martii

Autor(es):Mostrar menos -

Queiroz, Marcos Marcal Ferreira ^[1] ; Monteillier, Aymeric ^[1] ; Berndt, Sarah ^[1] ; Marcourt, Laurence ^[1] ; Franco, Eryvelton de Souza ^[2] ; Carpentier, Gilles ^[3] ; Ebrahimi, Samad Nejad ^[4] ; Cuendet, Muriel ^[1] ; Bolzani, Vanderlan da Silva ^[5] ; Souza Maia, Maria Bernadete ^[2] ; Queiroz, Emerson Ferreira ^[1] ; Wolfender, Jean-Luc ^[1]

Afiliação do(s) autor(es)

^[1] Univ Lausanne, Univ Geneva, Sch Pharmaceut Sci, Rue Michel Servet 1, CH-1211 Geneva 4 - Switzerland

^[2] Univ Fed Pernambuco, UFPE, Pharmacol Bioact Prod, BR-50670901 Recife, PE - Brazil

^[3] Univ Paris Est Creteil, Fac Sci & Technol, Lab CRRET, F-94010 Creteil - France

^[4] Shahid Beheshti Univ, Dept Phytochem, Med Plants & Drugs Res Inst, GC, Tehran 1983963113 - Iran

^[5] Univ Estadual Paulista, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, NuBBE, Inst Quim, BR-14800900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Natural Products; v. 81, n. 8, p. 1769-1776, AUG 2018.
• Citações Web of Science: 1

Resumo

The ethyl acetate extract of the aerial parts of Chresta martii showed significant in vitro NF-kappa B inhibition. Bioactivity-guided isolation was undertaken using HPLC microfractionation to localize the active compounds. Different zones of the HPLC chromatogram were linked to NF-kappa B inhibition. In parallel to this HPLC-based activity profiling, HPLC-PDA-ESI-MS and UHPLC-TOF-HRMS were used for the early identification of some of the compounds present in the extract and to get a complete phytochemical overview. The isolation of the compounds was performed by high-speed counter-current chromatography and further semipreparative HPLC. Using this approach, 14 compounds were isolated, two of them being new sesquiterpene lactones. The structures of the isolated compounds were elucidated by spectroscopic methods including UV, ECD, NMR, and HRMS. All isolated compounds were evaluated for their inhibitory activity of NF-kappa B and angiogenesis, and compound 2 showed promising NF-kappa B inhibition activity with an IC50 of 0.7 mu M. The isolated compounds 1, 2, 5, 7, and 8 caused a significant reduction in angiogenesis when evaluated by an original 3D in vitro angiogenesis assay. (AU)

• Processo FAPESP: 10/09780-0 - Uso da espectrometria de massas e da ressonância magnética nuclear para avaliação metabolômica & Identificação de protótipos inibidores de AChE em Banisteriopsis variabilis(B. Gates)- Malpighiaceae
• Beneficiário: Marcos Marçal Ferreira Queiroz
• Modalidade de apoio: Bolsas no Brasil - Doutorado