Subcellular localization and expression of E-cadherin and SNAIL are relevant since early stages of oral carcinogenesis

Autor(es)

Lopes, Nathalia Martins ^[1] ; Aquino Xavier, Flavia Calo ^[2] ; Ortiz, Rafael Carneiro ^[1] ; Amor, Nadia Ghinelli ^[1] ; Garlet, Gustavo Pompermaier ^[1] ; Lara, Vanessa Soares ^[3] ; Batista, Aline Carvalho ^[4] ; Costa, Nadia Lago ^[4] ; Rodini, Camila Oliveira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Fed Bahia, Lab Oral Surg Pathol, Sch Dent, Araujo Pinho Ave 62, 9th Floor, BR-40110150 Salvador, BA - Brazil

^[3] Univ Sao Paulo, Dept Surg Stomatol Pathol & Radiol, Bauru Sch Dent, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[4] Univ Fed Goias, Sch Dent, Dept Oral Med Oral Pathol Stomatol & Radiol, Praca Univ S-N Campus 1, 2nd Floor, BR-74605220 Goiania, Go - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PATHOLOGY RESEARCH AND PRACTICE; v. 214, n. 8, p. 1185-1191, AUG 2018.
• Citações Web of Science: 0

Resumo

The biological process of epithelial-to-mesenchymal transition (EMT) has been studied in oral squamous cell carcinoma (OSCC) metastasis, but it is rarely evaluated at several stages of oral carcinogenesis. This study aimed to analyze the presence of SNAIL and E-cadherin proteins, markers of EMT, in the development and progression of OSCC, evaluating excised specimens of potentially malignant lesions (oral leukoplakia with and without dysplasia-OL and OLD, respectively), tumor tissues (OSCC), metastatic lymph nodes (LN), and normal oral mucosa (NOM) by immunohistochemistry, considering subcellular localization. Additionally, SNAIL and Ecadherin transcripts were evaluated in vitro by qPCR, using SCC-9 cell line in comparison to human keratinocytes (HPEC). There was a significant increase in nuclear expression of SNAIL from NOM to OLD followed by a noticeable decrease in nuclear expression accompanied by increased cytoplasmic expression in OSCC (p < 0.05). The E-cadherin cytoplasmic expression was remarkable and statistically significant higher in OSCC and LN, both compared to NOM (p < 0.0001), OL (p < 0.01) and OLD (p < 0.0001 and p < 0.001, respectively). In vitro, Ecadherin and SNAIL transcripts were lower in SCC-9 compared to HPEC cells, although only the decrease of Ecadherin was statistically significant (p < 0.05). Regarding the association of E-cadherin and SNAIL expression with the clinical findings, the analysis revealed an association between the cytoplasmic expression of SNAIL and the invasion pattern (p = 0.05) in OSCC. The increased nuclear SNAIL expression may be characteristic of OLD, and the presence of E-cadherin in cell cytoplasm a marker of transformation to malignancy of potentially malignant oral leukoplakias into OSCC. (AU)

• Processo FAPESP: 13/01042-9 - Avaliação imuno-histoquímica da expressão dos marcadores de transição epitélio-mesenquimal E/N-caderina e SNAIL/SLUG na seqüência epitélio displásico - Carcinoma epidermóide de boca - metástase linfonodal
• Beneficiário: Nathália Martins Lopes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica