Glucose tolerant and glucose stimulated beta-glucosidases - A review

Autor(es)

Santos Salgado, Jose Carlos ^[1] ; Meleiro, Luana Parras ^[1] ; Carli, Sibeli ^[1] ; Ward, Richard John ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Bioresource Technology; v. 267, p. 704-713, NOV 2018.
• Citações Web of Science: 15

Resumo

The beta-glucosidases (beta-D-glucoside glucohydrolase, EC 3.2.1.21) hydrolyze glycosidic bonds of alkyl-, amino-, or aryl-beta-D-glucosides, cyanogenic glucosides, disaccharides and short oligosaccharides and can also catalyze the synthesis of glycosyl-bonds between different molecules via transglycosylation. Due to their ubiquitous phylogenetic distribution, substrate diversity and ability to both hydrolyze and synthesize glycosidic bonds, the catalysis and regulation of beta-glucosidases have been extensively studied. Many beta-glucosidases are inhibited by the reaction product glucose, and reduced catalytic activity may limit the biotechnological and industrial applications of these enzymes and this has stimulated the search for beta-glucosidases that maintain their activity at high glucose concentrations. Studies of many glucose tolerant enzymes have been reported and due to the ongoing interest in these enzymes, here it has been reviewed this accumulated body of knowledge which provides valuable insights as to the kinetics, structure, regulation and evolution of glucose tolerant and glucose stimulated beta-glucosidases. (AU)

• Processo FAPESP: 17/13734-3 - Engenharia de uma endopoligalacturonase por inserção de diferentes CBMs (Carbohydrate Binding Modules): quimerogênese e efeito de proximidade
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• Modalidade de apoio: Bolsas no Brasil - Doutorado
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• Processo FAPESP: 16/17582-0 - Modulação da termoestabilidade de uma ß-glucosidase estimulada por glicose e xilose empregando técnicas de glicosilação sítio dirigida
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