Potential prophylactic effect of recombinant bovine somatotropin (rbST) in sheep with experimentally induced hyperketonemia

Autor(es)

Araujo, Carolina A. S. C. ^[1] ; Sousa, Rejane S. ^[2] ; Monteiro, Bruno M. ^[3] ; Oliveira, Francisco L. C. ^[2] ; Minervino, Antonio H. H. ^[4] ; Rodrigues, Frederico A. M. L. ^[2] ; Vale, Rodolfo G. ^[2] ; Mori, Clara S. ^[2] ; Ortolani, Enrico L. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Rural Pernambuco UFRPE, Dept Vet Med, Dom Manuel de Medeiros St, Recife, PE - Brazil

^[2] Univ Sao Paulo, Dept Vet Med Clin, Fac Vet Med & Anim Sci FMVZ, Orlando Marques de Paiva Av 87, Cidade Univ, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dept Anim Reprod, Fac Vet Med & Anim Sci FMVZ, Orlando Marques de Paiva Av 87, Cidade Univ, Sao Paulo, SP - Brazil

^[4] Univ Fed Oeste Para LARSANA UFOPA, Lab Sanidade Anim, Rua Vera Paz S-N, Santarem, Para - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Research in Veterinary Science; v. 119, p. 215-220, AUG 2018.
• Citações Web of Science: 0

Resumo

Hyperketonemia in sheep is associated with a disease known as pregnancy toxemia. The purposes of this study were to (1) induce hyperketonemia by infusion with D-beta-hydroxybutyrate (D-BHB) in eighteen healthy, nonpregnant, non-lactating ewes, (2) to evaluate the metabolic pathways in which recombinant bovine somatotropin (rbST) may be involved, and (3) to evaluate the possible benefits of preventive rbST therapy on the metabolic profiles of the sheep. The sheep were intravenously infused with a D-BHB solution over 2 h. Three days prior to the infusion, one group (n = 9) was injected with a single dose of 160 mg rbST, and the other group (n = 9) was injected with saline as a control. Blood samples were collected and metabolic profiles were analyzed every 20 min during the infusion and at 15, 30, 60, 120, 180, and 240 min post-infusion. Hyperketonemia was successfully achieved, demonstrated by serum BHB concentrations of 3.9 and 3.0 mmol/L in the rbST and control groups, respectively, without the presence of clinical signs. An increase in peripheral insulin resistance was observed after infusion but not during the infusion, in the rbST group. Higher glucose concentrations were observed in the rbST group post-infusion, suggesting a positive impact of D-BHB on gluconeogenesis. Our data suggest that prophylactic treatment with rbST may be useful in sheep to reduce the risk of PT. (AU)

• Processo FAPESP: 11/20101-0 - Perfil metabólico de ovelhas submetidas à hipertriacilgliceremia e tratadas metafiláticamente com somatotropina recombinante bovina (r-bST)
• Beneficiário: Enrico Lippi Ortolani
• Modalidade de apoio: Auxílio à Pesquisa - Regular