Changes in the expression of matrix extracellular genes and TGFB family members in rotator cuff tears

Autor(es):Mostrar menos -

Belangero, Paulo Santoro ^[1] ; Figueiredo, Eduardo Antonio ^[1] ; Cohen, Carina ^[1] ; Alves, Felipe de Seixas ^{[1, 2]} ; Yanaguizawa, Wania Hiromi ^{[1, 2]} ; Smith, Marilia Cardoso ^[3] ; Andreoli, Carlos Vicente ^[1] ; Pochini, Alberto de Castro ^[1] ; de Seixas Alves, Maria Teresa ^[2] ; Ejnisman, Benno ^[1] ; Cohen, Moises ^[1] ; Leal, Mariana Ferreira ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Ortopedia & Traumatol, Rua Borges Lagoa 783, BR-04038032 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Patol, BR-04038032 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Morfol Genet, Disciplina Genet, BR-04023001 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORTHOPAEDIC RESEARCH; v. 36, n. 9, p. 2542-2553, SEP 2018.
• Citações Web of Science: 0

Resumo

Lack of synthesis of extracellular matrix compounds may contribute to degeneration of the tendons. Thus, we aimed to evaluate the expression of extracellular matrix and TGFB family members in ruptured and non-ruptured tendons of the rotator cuff, as well as the effect of clinical factors on gene expression in tendon samples, and the relationship between histological findings and altered gene expression. Injured and non-injured supraspinatus tendon samples and subscapular non-injured tendon samples were collected from 38 patients with rotator cuff tears. Non-injured supraspinatus tendons were obtained from eight controls. Specimens were used for histological evaluation, quantification of collagen fibers, and mRNA and protein expression analyses. Increased COL1A1, COL1A2, COL3A1, COL5A1, FN1, TNC, and TGFBR1 mRNA expression was observed in the tear samples (p<0.05). Duration of symptoms was correlated with the levels of collagen type I/III fibers (p=0.032; =0.0447) and FN1 immunostaining (p=0.031; =0.417). Smoking was associated with increased frequency of microcysts, myxoid degeneration, and COL5A1, FN1, TNC, and TGFB1 mRNA expression (p<0.05). FN1 immunostaining was correlated with the number of years of smoking (p=0.048; =0.384). Lower levels of collagen type I/III fibers were detected in samples with fissures (0=0.046). High frequency of microcysts was associated with increased COL5A1, FN1, and TNC expression (p<0.05, for all comparisons). Neovascularization was associated with reduced FN1 (p=0.035) and TGFBR1 expression (p=0.034). Our findings show differential expression of matrix extracellular genes and TGFB family members in the degeneration process involved in rotator cuff tears. These molecular alterations are influenced by clinical factors. (c) 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2542-2553, 2018. (AU)

• Processo FAPESP: 11/22548-2 - Afecções ortopédicas não traumáticas de ombro: aspectos genéticos e moleculares
• Beneficiário: Mariana Ferreira Leal
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/14768-5 - Afecções ortopédicas não traumáticas de ombro: aspectos genéticos e moleculares
• Beneficiário: Mariana Ferreira Leal
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores
• Processo FAPESP: 13/10791-5 - Análise histológica e da imunorreatividade de proteínas de matriz extracelular em lesões ortopédicas relacionadas à prática esportiva
• Beneficiário: Wânia Hiromi Yanaguizawa
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 13/10691-0 - Análise histológica e da expressão proteica de colágenos em lesão do ligamento cruzado anterior
• Beneficiário: Felipe de Seixas Alves
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica