Alveolar macrophage functions during the transition phase to active immunity in calves

Autor(es)

Bertagnon, Heloisa G. ^{[1, 2]} ; Batista, Camila F. ^[1] ; Santos, Kamila R. ^[1] ; Gomes, Renata C. ^[1] ; Bellinazzi, Jessyca B. ^[1] ; Della Libera, Alice Maria M. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Internal Med, BR-05508270 Sao Paulo, SP - Brazil

^[2] Univ Ctr Oeste Parana UNICENTRO, Dept Vet Med, BR-85040080 Guarapuava, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ANIMAL SCIENCE; v. 96, n. 9, p. 3738-3747, SEP 2018.
• Citações Web of Science: 1

Resumo

The first 3 to 6 mo of the life of calves is the period during which active immunity is established. During this period, greater morbidity and mortality is caused by bronchopneumonia because of the immaturity of the pulmonary immune system or the exaggerated cytotoxic response at subsequent infection. The aim of this study was to examine the maturity of the immune system during this phase of activation of acquired immunity in calves. For this purpose, the functions of phagocytosis and the reactive oxygen species (ROS) of alveolar macrophages CD14+ were evaluated. Further, the classes of immunoglobulins and the cytokines implicated in lymphocyte response patterns Th1 and Th2 in 10 healthy Holstein calves were quantified. Samples were taken from calves every 15 d, from the third to the sixth month of life. The alveolar macrophage CD14+ functions increased progressively until 150 d of age (phagocytosis, P = 0.02, ROS, P = 0.05), IgG1 and IgG2 isotype secretion reached an equilibrium, and the cytokine profiles were compatible with the Th1 response. At 165 d of age, there was a decrease in cellular function (phagocytosis P = 0.02, ROS P = 0.04) and an increase in IgG1 titers (P = 0.005) and IL-10 mRNA expression (P = 0.09). At 180 d of life, we observed an IgG1 and IgG2 secretion balance, a decrease in IL-10 mRNA expression, and an increase in IL-12 mRNA (P = 0.04) and tumor necrosis factor (TNF)-alpha mRNA expressions (P = 0.0003) and alveolar macrophage oxidative metabolism were observed. These results indicate that the calves had an active immune response that was distinctive for the age group. The CD14+ response is more reactive at 150 d. A regulatory and/or humoral response begins at 165 d of life as the equilibrium of Th1 and Th2 profiles is reached at 180 d of life. This may be clinically relevant for the development of specific therapies and prophylactic measures for bronchopneumonia in calves at 135 to 180 d of life. (AU)

• Processo FAPESP: 11/17606-3 - Avaliação da resposta imune inata respiratória em bezerros sadios durante o segundo trimestre de vida
• Beneficiário: Alice Maria Melville Paiva Della Libera
• Modalidade de apoio: Auxílio à Pesquisa - Regular