Acidic Dressing Based on Agarose/Cs2.5H0.5PW12O40 Nanocomposite for Infection Control in Wound Care

Autor(es)

Piva, Roger H. ^[1] ; Rocha, Marina C. ^[2] ; Piva, Diogenes H. ^[3] ; Imasato, Hidetake ^[1] ; Malavazi, Iran ^[2] ; Rodrigues-Filho, Ubirajara P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, Grp Quim Mat Hibridos & Inorgan, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Res Ctr Adv Mat & Energy, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 10, n. 37, p. 30963-30972, SEP 19 2018.
• Citações Web of Science: 3

Resumo

Regulation of wound pH from alkaline to acidic is a simple and powerful approach to reduce wound microbial colonization and infection. Here, we present a nanocomposite material possessing intrinsic acidic surface pH as an innovative antimicrobial wound dressing. This material comprises an agarose matrix nanocomposite containing nanoparticles (NPs) of the cesium salt of phosphotungstic heteropolyacid (Cs2.5H0.5-PW12O40), Self-supporting films were prepared by a casting method incorporating 5-20 wt % Cs2.5H0.5PW12O40 NPs into the matrix. Films are flexible with tensile strengths between 28.55 and 32.15 MPa and exhibit broad biocidal activity against neutralophilic pathogens, including Gram-positive bacteria, Gram-negative bacteria, yeast, and filamentous fungi. The nano antimicrobial Cs2.5H0.5PW12O40 functions as an efficient and selfcontrolled proton delivery agent that lowers the surface pH of the nanocomposites to the range 7.0 > pH >= 3.0. Nanocomposite films containing 20 wt % Cs2.5H0.5PW12O40 NPs presented a surface pH of 3.0 and highest antimicrobial activity. Using quantitative reverse transcription polymerase chain reaction, we demonstrated that the antimicrobial mechanism of the nanocomposites is acid-induced because of the transcriptional induction of glutamate-dependent acid resistance genes in Escherichia coli. Additionally, nanocomposite films do not damage skin according to an in vivo rabbit skin model with no derived edema or erythema. The wound care safety of this material is due to low release of heavy metal heteropolyanions ({[}PW12O40](3-)), no nanoparticle leaching, and proton controlled release resulting in nonirritating acid levels for human skin models. (AU)

• Processo FAPESP: 15/17541-0 - Estudo da relação da via da integridade da parede celular e de genes de resposta ao estresse térmico no fungo patogênico humano Aspergillus fumigatus
• Beneficiário: Iran Malavazi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/16098-8 - Superfícies antimicrobianas bioinspiradas no manto ácido humano utilizando heteropoliácidos tipo Keggin
• Beneficiário: Roger Honorato Piva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/00315-5 - A influência de proteínas quinases ativadas por mitógenos (MAPK) na expressão de determinantes genéticos importantes para a virulência de Aspergillus fumigatus
• Beneficiário: Marina Campos Rocha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/19694-3 - Identificação do papel das proteínas de choque térmico HsfA e Hsp90 e do regulador SmiA na atividade da via da integridade da parede celular no fungo patogênico oportunista humano Aspergillus fumigatus
• Beneficiário: Iran Malavazi
• Modalidade de apoio: Auxílio à Pesquisa - Regular