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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Acidic Dressing Based on Agarose/Cs2.5H0.5PW12O40 Nanocomposite for Infection Control in Wound Care

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Piva, Roger H. [1] ; Rocha, Marina C. [2] ; Piva, Diogenes H. [3] ; Imasato, Hidetake [1] ; Malavazi, Iran [2] ; Rodrigues-Filho, Ubirajara P. [1]
Total Authors: 6
[1] Univ Sao Paulo, Inst Quim Sao Carlos, Grp Quim Mat Hibridos & Inorgan, BR-13563120 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, BR-13565905 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Res Ctr Adv Mat & Energy, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ACS APPLIED MATERIALS & INTERFACES; v. 10, n. 37, p. 30963-30972, SEP 19 2018.
Web of Science Citations: 2

Regulation of wound pH from alkaline to acidic is a simple and powerful approach to reduce wound microbial colonization and infection. Here, we present a nanocomposite material possessing intrinsic acidic surface pH as an innovative antimicrobial wound dressing. This material comprises an agarose matrix nanocomposite containing nanoparticles (NPs) of the cesium salt of phosphotungstic heteropolyacid (Cs2.5H0.5-PW12O40), Self-supporting films were prepared by a casting method incorporating 5-20 wt % Cs2.5H0.5PW12O40 NPs into the matrix. Films are flexible with tensile strengths between 28.55 and 32.15 MPa and exhibit broad biocidal activity against neutralophilic pathogens, including Gram-positive bacteria, Gram-negative bacteria, yeast, and filamentous fungi. The nano antimicrobial Cs2.5H0.5PW12O40 functions as an efficient and selfcontrolled proton delivery agent that lowers the surface pH of the nanocomposites to the range 7.0 > pH >= 3.0. Nanocomposite films containing 20 wt % Cs2.5H0.5PW12O40 NPs presented a surface pH of 3.0 and highest antimicrobial activity. Using quantitative reverse transcription polymerase chain reaction, we demonstrated that the antimicrobial mechanism of the nanocomposites is acid-induced because of the transcriptional induction of glutamate-dependent acid resistance genes in Escherichia coli. Additionally, nanocomposite films do not damage skin according to an in vivo rabbit skin model with no derived edema or erythema. The wound care safety of this material is due to low release of heavy metal heteropolyanions ({[}PW12O40](3-)), no nanoparticle leaching, and proton controlled release resulting in nonirritating acid levels for human skin models. (AU)

FAPESP's process: 16/16098-8 - Antimicrobial surfaces bionspired by the acid mantle based on Keggin-type heteropoly acids
Grantee:Roger Honorato Piva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/17541-0 - Study of the relationship between genes involved in the cell wall integrity maintenance and thermotolerance in the human pathogenic fungi Aspergillus fumigatus
Grantee:Iran Malavazi
Support type: Regular Research Grants
FAPESP's process: 18/00315-5 - The influence of mitogen activated protein kinases (MAPK) on the expression of genetic determinants important for Aspergillus fumigatus virulence
Grantee:Marina Campos Rocha
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/19694-3 - The role of heat shock proteins hsfA and Hsp90 and the regulator SmiA at cell wall integrity pathway activity in the opportunistic human pathogen Aspergillus fumigatus
Grantee:Iran Malavazi
Support type: Regular Research Grants