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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Controlling methylene blue aggregation: a more efficient alternative to treat Candida albicans infections using photodynamic therapy

Texto completo
Autor(es):
da Collina, Gabriela Alves [1] ; Freire, Fernanda [1] ; da Costa Santos, Thabata Paulino [1] ; Sobrinho, Natalia Gesse [1] ; Aquino, Simone [2] ; Prates, Renato Araujo [1] ; Teixeira da Silva, Daniela de Fatima [1] ; Ratto Tempestini Horliana, Anna Carolina [1] ; Pavani, Christiane [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo - Brazil
[2] Univ Nove Julho UNINOVE, Profess Masters Program Adm Hlth Syst Management, Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 17, n. 10, p. 1355-1364, OCT 1 2018.
Citações Web of Science: 4
Resumo

Methylene Blue (MB) has been widely used in antimicrobial Photodynamic Therapy (aPDT), however, the mechanisms of action (Type I or Type II) are defined by its state of aggregation. In this sense, the identification of the relationships between aggregation, the mechanisms of action and the effectiveness against microorganisms, as well as the establishment of the means and the formulations that may favor the most effective mechanisms, are essential. Thus, the objective of this study was to assess the in vitro aPDT efficacies against Candida albicans, by using MB in vehicles which may influence the aggregation and present an oral formulation (OF) containing MB, to be used in clinical aPDT procedures. The efficacy of MB at 20 mg L-1 was tested in a range of vehicles (water, physiological solution - NaCl 0.9%, phosphate saline buffer - PBS, sodium dodecyl sulfate 0.25% - SDS and urea 1 mol L-1) in a C. albicans planktonic culture, when using 4.68 J cm(-2) of 640 +/- 12 nm LED for the irradiations, as well as 5 minutes of pre-irradiation time, together with measuring the UFC mL(-1). Based upon these analyses, an OF containing MB in the most effective vehicle was tested in the biofilms, as a proposal for clinical applications. When comparing some of the vehicles, sodium dodecyl sulfate was the only one that enhanced an MB aPDT efficacy in a planktonic C. albicans culture. This OF was tested in the biofilms and 50 mg L-1 MB was necessary, in order to achieve some reduction in the cell viabilities after the various treatments. The light dosimetries still need further adaptations, in order for this formulation to be used in clinical applications. The present research has indicated that the development of this formulation for the control of MB aggregations may result in more effective clinical protocols. (AU)

Processo FAPESP: 16/13488-0 - Influência do meio na eficiência fotodinâmica de azul de metileno na intativação de Candida Albicans
Beneficiário:Natalia Gesse Sobrinho
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 16/03037-0 - Agregação de fotossensibilizadores e suas implicações nos mecanismos fotoquímicos e na efetividade da terapia fotodinâmica
Beneficiário:Christiane Pavani
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/50680-5 - Fotossensibilização nas ciências da vida
Beneficiário:Mauricio da Silva Baptista
Linha de fomento: Auxílio à Pesquisa - Temático