Evidence of photoinduced lipid hydroperoxidation in Langmuir monolayers containing Eosin Y

Autor(es)

Pereira, Lucas S. A. ^[1] ; Camacho, Sabrina A. ^[1] ; Malfatti-Gasperini, Antonio A. ^[2] ; Jochelavicius, Karen ^[3] ; Nobre, Thatyane M. ^[3] ; Oliveira, Jr., Osvaldo N. ^[3] ; Aoki, Pedro H. B. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Sci Humanities & Languages, BR-19806900 Assis, SP - Brazil

^[2] CNPEM, Brazilian Synchrotron Light Lab LNLS, Rua Giuseppe Maximo Scolfaro 10000, BR-13083070 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Phys, IFSC, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 171, p. 682-689, NOV 1 2018.
• Citações Web of Science: 4

Resumo

Photodynamic therapy (PDT) efficiency depends on many factors including the incorporation of the photo sensitizer (PS) in cell membranes and possible lipid hydroperoxidation. In this study, we show that hydroperoxidation may be photoinduced when eosin Y is incorporated into Langmuir monolayers that serve as cell membrane models. This occurs for Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), which have unsaturation in their hydrophobic chains. In contrast, light irradiation had no effect on monolayers of saturated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). Evidence of hydroperoxidation was obtained from the area increase in eosin-containing DOPC and POPC monolayers upon irradiation, which was accompanied by a decrease in monolayer thickness according to grazing incidence X-ray off-specular scattering (GIXOS) data. Furthermore, the changes in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) induced by irradiation were consistent with hydroperoxide migration toward the lipid hydrophilic heads.. In summary, this combination of experimental methods allowed us to determine the effects of eosin Y interaction with cell membrane models under irradiation, which may be associated with the underlying mechanisms of eosin Y as photosensitizer in PDT. (AU)

• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/13280-0 - Mecanismos moleculares da fotossensibilização de membranas lipídicas e seus efeitos em terapia fotodinâmica
• Beneficiário: Pedro Henrique Benites Aoki
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/13404-3 - Foto-oxidação lipídica mediada pelo xantênico eosina Y em sistemas modelos baseados em filmes de Langmuir
• Beneficiário: Lucas Sant Anna Pereira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica