Control of bacterial biofilms formed on pacifiers by antimicrobial solutions in spray

Autor(es)

Pedroso, Juliana de F. ^[1] ; Sangalli, Jorgiana ^[1] ; Brighenti, Fernanda L. ^[2] ; Tanaka, Marcia H. ^[1] ; Koga-Ito, Cristiane Yumi ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol Campus Sao Jose dos Campos, Oral Biopathol Grad Program, Sao Jose Dos Campos, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Sci & Technol Campus Sao Jose dos Campos, Dept Environm Engn, Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Paediatric Dentistry; v. 28, n. 6, p. 578-586, NOV 2018.
• Citações Web of Science: 0

Resumo

Background. The aim was to evaluate the use of vinegar, hydrogen peroxide and sodium bicarbonate solutions for the disinfection of pacifiers. Design. Different types and concentrations of the substances were screened in planktonic and bio-film for antimicrobial activity on Streptococcus mutans,.Staphylococcus pyogenes, Staphylococcus aureus and Escherichia coli. Also, the effect of the most effective substance was tested against polymicrobial biofilms aiming to mimic the multispccies colonization found in a pacifier. Results. Based on the agar diffusion test, minimal inhibitory concentration (MIC) and minimal microbicide concentration assays, 70% apple vinegar (70%AV) and 70% hydrogen peroxide (70%HP) were selected (P < 0.05). All tested solutions were able to significantly reduce viable cells of S. pyogenes, S. mutans and E. coil from the biofilms A statistically significant reduction of viable cells in S. aureus biofilms were observed after treatment with 70%AV in comparison to the other treatments. When compared to other microbial species, it was found that 70%HP was particularly effective against E. colt. The solution of 70% HP was able to reduce the viable cells of Enterobacteriaceae/pseudomonas of a polymicrobial biofilm formed from a pacifier. The other solutions were not effective in relation to control. group. Conclusion. Solutions of 70% HP showed to be a good alternative for chlorhexidine digluconate for the disinfection of pacifiers. (AU)

• Processo FAPESP: 12/10891-7 - Eficácia de soluções alternativas em spray na desinfecção de chupetas contaminadas por biofilme de microcosmo
• Beneficiário: Juliana de Fátima Pedroso
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica