Effect of bone morphogenetic protein 9 on osteoblast differentiation of cells grown on titanium with nanotopography

Autor(es)

Souza, Alann T. P. ^[1] ; Bezerra, Barbara L. S. ^[1] ; Oliveira, Fabiola S. ^[1] ; Freitas, Gileade P. ^[1] ; Bighetti Trevisan, Rayana L. ^[1] ; Oliveira, Paulo T. ^[1] ; Rosa, Adalberto L. ^[1] ; Beloti, Marcio M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Biochemistry; v. 119, n. 10, p. 8441-8449, NOV 2018.
• Citações Web of Science: 3

Resumo

Among bone morphogenetic proteins (BMPs), BMP-9 has been described as one with higher osteogenic potential. Here, we aimed at evaluating the effect of BMP-9 on the osteoblast differentiation of cells grown on titanium (Ti) with nanotopography, a well-known osseoinductive surface. MC3T3-E1 cells were grown either in absence or presence of BMP-9 (20 nM) on Ti with nanotopography (Ti-Nano) or machined Ti (Ti-Machined) for up to 21 days to evaluate the gene expression of RUNX2, osterix, osteocalcin, bone sialoprotein, SMAD6 and SMAD4, protein expression of SMAD4, ALP activity and extracellular matrix mineralization. As expected BMP-9 increased osteoblast differentiation irrespective of Ti surface topography; however, the cells grown on Ti-Nano were more responsible to BMP-9 compared with cells grown on Ti-machined. This could be, at least in part, due to the fact that Ti-Nano may act on both ways, by increasing the activation (SMAD4) and decreasing the inhibition (SMAD6) of the signaling pathway triggered by BMP-9, while Ti-Machined only decrease the inhibition (SMAD6) of this pathway. In conclusion, the combination of the osteogenic potential of BMP-9 with the osseoinductive capacity of Ti-Nano could be a promising strategy to favor the osseointegration of Ti implants. (AU)

• Processo FAPESP: 16/14171-0 - Papel das Vias de Sinalização de Wnt no Potencial Osteogênico de Superfície de Titânio com Nanotopografia
• Beneficiário: Márcio Mateus Beloti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/14477-1 - Participação da proteína óssea morfogenética 9 (BMP-9) na interação entre osteoblastos e superfície de titânio com nanotopografia
• Beneficiário: Barbara Luana da Silva Bezerra
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica